CRISPR-U™ Gene Knockout Cell Line
Strategy
MELK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MELK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MELK |
| Gene id | 9833 |
| Organism | Homo sapiens |
| Official full symbol | maternal embryonic leucine zipper kinase |
| Gene type | protein-coding |
| Also known as | HPK38 |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MELK-201 | ENST00000298048.7 | 2452 | 651aa | Protein coding | CCDS6606 | Q14680-1 | NM_014791.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MELK-210 | ENST00000543751.5 | 2400 | 619aa | Protein coding | CCDS59126 | Q14680-6 | - | TSL:1, GENCODE basic, |
| MELK-209 | ENST00000541717.4 | 2363 | 610aa | Protein coding | CCDS59123 | Q14680-7 | - | TSL:2, GENCODE basic, |
| MELK-207 | ENST00000536860.5 | 2342 | 603aa | Protein coding | CCDS59124 | Q14680-8 | - | TSL:2, GENCODE basic, |
| MELK-206 | ENST00000536329.5 | 2283 | 580aa | Protein coding | CCDS59127 | Q14680-5 | - | TSL:1, GENCODE basic, |
| MELK-211 | ENST00000545008.5 | 2236 | 580aa | Protein coding | CCDS59125 | Q14680-2 | - | TSL:2, GENCODE basic, |
| MELK-208 | ENST00000536987.5 | 2125 | 520aa | Protein coding | CCDS59128 | Q14680-4 | - | TSL:2, GENCODE basic, |
| MELK-205 | ENST00000495529.5 | 794 | 215aa | Protein coding | - | A0A0D9SGI4 | - | CDS 3' incomplete, TSL:5, |
| MELK-214 | ENST00000627766.2 | 2263 | 165aa | Nonsense mediated decay | - | A0A0D9SFB9 | - | TSL:5, |
| MELK-213 | ENST00000626154.2 | 1966 | 129aa | Nonsense mediated decay | - | A0A0D9SFF9 | - | TSL:2, |
| MELK-212 | ENST00000625974.2 | 918 | 157aa | Nonsense mediated decay | - | A0A0D9SFL8 | - | TSL:5, |
| MELK-203 | ENST00000487398.3 | 962 | No protein | Processed transcript | - | - | - | TSL:5, |
| MELK-204 | ENST00000489766.5 | 551 | No protein | Processed transcript | - | - | - | TSL:3, |
| MELK-202 | ENST00000480021.1 | 460 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MELK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MELK had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: