CRISPR-U™ Gene Knockout Cell Line Strategy

MELK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MELK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MELK
Gene id 9833
Organism Homo sapiens
Official full symbol maternal embryonic leucine zipper kinase
Gene type protein-coding
Also known as HPK38
Genomic regions Chromosome 9
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MELK-201 ENST00000298048.7 2452 651aa Protein coding CCDS6606 Q14680-1 NM_014791.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MELK-210 ENST00000543751.5 2400 619aa Protein coding CCDS59126 Q14680-6 - TSL:1, GENCODE basic,
MELK-209 ENST00000541717.4 2363 610aa Protein coding CCDS59123 Q14680-7 - TSL:2, GENCODE basic,
MELK-207 ENST00000536860.5 2342 603aa Protein coding CCDS59124 Q14680-8 - TSL:2, GENCODE basic,
MELK-206 ENST00000536329.5 2283 580aa Protein coding CCDS59127 Q14680-5 - TSL:1, GENCODE basic,
MELK-211 ENST00000545008.5 2236 580aa Protein coding CCDS59125 Q14680-2 - TSL:2, GENCODE basic,
MELK-208 ENST00000536987.5 2125 520aa Protein coding CCDS59128 Q14680-4 - TSL:2, GENCODE basic,
MELK-205 ENST00000495529.5 794 215aa Protein coding - A0A0D9SGI4 - CDS 3' incomplete, TSL:5,
MELK-214 ENST00000627766.2 2263 165aa Nonsense mediated decay - A0A0D9SFB9 - TSL:5,
MELK-213 ENST00000626154.2 1966 129aa Nonsense mediated decay - A0A0D9SFF9 - TSL:2,
MELK-212 ENST00000625974.2 918 157aa Nonsense mediated decay - A0A0D9SFL8 - TSL:5,
MELK-203 ENST00000487398.3 962 No protein Processed transcript - - - TSL:5,
MELK-204 ENST00000489766.5 551 No protein Processed transcript - - - TSL:3,
MELK-202 ENST00000480021.1 460 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MELK
This KO Strategy loading
Red Cotton™ Notes Gene MELK had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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