CRISPR-U™ Gene Knockout Cell Line Strategy

PTDSS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTDSS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTDSS1
Gene id 9791
Organism Homo sapiens
Official full symbol phosphatidylserine synthase 1
Gene type protein-coding
Also known as LMHD, PSS1, PSSA
Summary The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 8
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTDSS1-202 ENST00000517309.6 4990 473aa Protein coding CCDS6271 P48651-1 NM_014754.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTDSS1-206 ENST00000522072.1 1112 301aa Protein coding - P48651-3 - TSL:2, GENCODE basic,
PTDSS1-201 ENST00000337004.8 2366 108aa Nonsense mediated decay - J3KNR6 - TSL:1,
PTDSS1-205 ENST00000518776.1 766 No protein Processed transcript - - - TSL:3,
PTDSS1-204 ENST00000517982.1 1215 No protein Retained intron - - - TSL:2,
PTDSS1-203 ENST00000517557.5 757 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTDSS1
This KO Strategy loading
Red Cotton™ Notes Gene PTDSS1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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