CRISPR-U™ Gene Knockout Cell Line Strategy

CD151 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CD151 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD151
Gene id 977
Organism Homo sapiens
Official full symbol CD151 molecule (Raph blood group)
Gene type protein-coding
Also known as GP27, MER2, PETA-3, RAPH, SFA1, TSPAN24
Summary The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD151-201 ENST00000322008.9 1545 253aa Protein coding CCDS7719 A0A024RCB3 P48509 - TSL:1, GENCODE basic, APPRIS P4,
CD151-202 ENST00000397420.9 1545 253aa Protein coding CCDS7719 A0A024RCB3 P48509 NM_004357.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CD151-203 ENST00000397421.5 1486 253aa Protein coding CCDS7719 A0A024RCB3 P48509 - TSL:1, GENCODE basic, APPRIS P4,
CD151-213 ENST00000528011.2 1425 251aa Protein coding - E9PMR4 - TSL:5, GENCODE basic, APPRIS ALT1,
CD151-204 ENST00000524748.6 980 230aa Protein coding - K4DIA7 - CDS 3' incomplete, TSL:5,
CD151-212 ENST00000527341.5 797 180aa Protein coding - K4DIB7 - CDS 3' incomplete, TSL:3,
CD151-211 ENST00000526693.5 771 176aa Protein coding - E9PLZ6 - CDS 3' incomplete, TSL:3,
CD151-209 ENST00000526439.1 713 64aa Protein coding - E9PP93 - CDS 3' incomplete, TSL:2,
CD151-217 ENST00000530320.5 648 170aa Protein coding - E9PJC8 - CDS 3' incomplete, TSL:3,
CD151-215 ENST00000529810.5 608 178aa Protein coding - E9PRJ3 - CDS 3' incomplete, TSL:3,
CD151-206 ENST00000525333.5 561 79aa Protein coding - E9PK37 - CDS 3' incomplete, TSL:4,
CD151-214 ENST00000528867.5 545 90aa Protein coding - E9PJE8 - CDS 3' incomplete, TSL:4,
CD151-207 ENST00000525718.6 534 82aa Protein coding - E9PSA1 - CDS 3' incomplete, TSL:2,
CD151-218 ENST00000530726.5 1407 253aa Nonsense mediated decay CCDS7719 A0A024RCB3 P48509 - TSL:5,
CD151-222 ENST00000642413.1 525 No protein Processed transcript - - - -
CD151-205 ENST00000525181.1 364 No protein Processed transcript - - - TSL:3,
CD151-223 ENST00000645878.1 1857 No protein Retained intron - - - -
CD151-220 ENST00000532045.6 1835 No protein Retained intron - - - TSL:5,
CD151-210 ENST00000526661.5 894 No protein Retained intron - - - TSL:2,
CD151-221 ENST00000532075.1 786 No protein Retained intron - - - TSL:2,
CD151-219 ENST00000531999.5 580 No protein Retained intron - - - TSL:3,
CD151-208 ENST00000525868.5 541 No protein Retained intron - - - TSL:4,
CD151-216 ENST00000530155.5 493 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD151
This KO Strategy loading
Red Cotton™ Notes Gene CD151 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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