CRISPR-U™ Gene Knockout Cell Line Strategy

CEP57 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CEP57 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CEP57
Gene id 9702
Organism Homo sapiens
Official full symbol centrosomal protein 57
Gene type protein-coding
Also known as MVA2, PIG8, TSP57
Summary This gene encodes a cytoplasmic protein called Translokin. This protein localizes to the centrosome and has a function in microtubular stabilization. The N-terminal half of this protein is required for its centrosome localization and for its multimerization, and the C-terminal half is required for nucleating, bundling and anchoring microtubules to the centrosomes. This protein specifically interacts with fibroblast growth factor 2 (FGF2), sorting nexin 6, Ran-binding protein M and the kinesins KIF3A and KIF3B, and thus mediates the nuclear translocation and mitogenic activity of the FGF2. It also interacts with cyclin D1 and controls nucleocytoplasmic distribution of the cyclin D1 in quiescent cells. This protein is crucial for maintaining correct chromosomal number during cell division. Mutations in this gene cause mosaic variegated aneuploidy syndrome, a rare autosomal recessive disorder. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 11
Strategy Summary
This gene has 9 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CEP57-202 ENST00000325542.10 3141 500aa Protein coding CCDS8304 Q86XR8-1 NM_014679.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CEP57-201 ENST00000325486.9 3098 474aa Protein coding CCDS58166 Q86XR8-2 - TSL:1, GENCODE basic, APPRIS ALT2,
CEP57-213 ENST00000541150.5 2911 491aa Protein coding CCDS58167 Q86XR8-5 - TSL:5, GENCODE basic, APPRIS ALT2,
CEP57-207 ENST00000537677.5 1647 473aa Protein coding CCDS86240 F5GYW0 - TSL:5, GENCODE basic, APPRIS ALT2,
CEP57-210 ENST00000538658.5 2084 270aa Protein coding - Q86XR8-3 - TSL:1, GENCODE basic,
CEP57-214 ENST00000541365.5 870 205aa Protein coding - F5H1B0 - CDS 3' incomplete, TSL:3,
CEP57-216 ENST00000544522.5 851 198aa Protein coding - F5H0C6 - CDS 3' incomplete, TSL:2,
CEP57-203 ENST00000535224.1 760 254aa Protein coding - H0YFT8 - CDS 5' and 3' incomplete, TSL:3,
CEP57-206 ENST00000537093.5 404 134aa Protein coding - H0YGT4 - CDS 5' and 3' incomplete, TSL:5,
CEP57-212 ENST00000540830.5 1936 71aa Nonsense mediated decay - F5GXS6 - TSL:1,
CEP57-211 ENST00000539855.5 1739 82aa Nonsense mediated decay - F5H6R3 - TSL:5,
CEP57-208 ENST00000538095.1 590 52aa Nonsense mediated decay - F5GZ93 - TSL:4,
CEP57-204 ENST00000535497.1 446 41aa Nonsense mediated decay - F5H7J4 - TSL:3,
CEP57-205 ENST00000536587.5 447 No protein Processed transcript - - - TSL:5,
CEP57-209 ENST00000538158.1 2632 No protein Retained intron - - - TSL:2,
CEP57-215 ENST00000541768.1 503 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CEP57
This KO Strategy loading
Red Cotton™ Notes Gene CEP57 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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