CRISPR-U™ Gene Knockout Cell Line
Strategy
BZW1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BZW1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BZW1 |
| Gene id | 9689 |
| Organism | Homo sapiens |
| Official full symbol | basic leucine zipper and W2 domains 1 |
| Gene type | protein-coding |
| Also known as | BZAP45, Nbla10236 |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BZW1-203 | ENST00000409600.6 | 6511 | 419aa | Protein coding | CCDS56156 | Q7L1Q6-1 | NM_001207067.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BZW1-202 | ENST00000409226.5 | 2455 | 423aa | Protein coding | CCDS56154 | Q7L1Q6-4 | - | TSL:2, GENCODE basic, |
| BZW1-209 | ENST00000452790.6 | 1473 | 451aa | Protein coding | CCDS56155 | Q7L1Q6-3 | - | TSL:2, GENCODE basic, |
| BZW1-204 | ENST00000410110.6 | 1075 | 294aa | Protein coding | - | C9IZ80 | - | CDS 3' incomplete, TSL:5, |
| BZW1-208 | ENST00000452206.5 | 1059 | 216aa | Protein coding | - | C9JFN4 | - | CDS 3' incomplete, TSL:5, |
| BZW1-201 | ENST00000359893.4 | 779 | 135aa | Protein coding | - | H0Y503 | - | CDS 5' incomplete, TSL:3, |
| BZW1-207 | ENST00000450637.5 | 578 | 154aa | Protein coding | - | C9J188 | - | CDS 3' incomplete, TSL:4, |
| BZW1-205 | ENST00000419090.1 | 557 | 161aa | Protein coding | - | C9JWF5 | - | CDS 3' incomplete, TSL:3, |
| BZW1-206 | ENST00000447069.5 | 557 | 116aa | Protein coding | - | C9JV57 | - | CDS 3' incomplete, TSL:4, |
| BZW1-211 | ENST00000463310.1 | 582 | No protein | Retained intron | - | - | - | TSL:2, |
| BZW1-212 | ENST00000464483.1 | 530 | No protein | Retained intron | - | - | - | TSL:2, |
| BZW1-213 | ENST00000491576.1 | 492 | No protein | Retained intron | - | - | - | TSL:2, |
| BZW1-210 | ENST00000460660.1 | 265 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BZW1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BZW1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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