CRISPR-U™ Gene Knockout Cell Line Strategy

CD58 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CD58 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD58
Gene id 965
Organism Homo sapiens
Official full symbol CD58 molecule
Gene type protein-coding
Also known as LFA-3, LFA3, ag3
Summary This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD58-203 ENST00000457047.6 1328 248aa Protein coding CCDS44199 P19256-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CD58-202 ENST00000369489.10 1086 250aa Protein coding CCDS888 P19256-1 NM_001779.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CD58-201 ENST00000369487.3 892 240aa Protein coding - B1AMW1 - TSL:1, GENCODE basic, APPRIS ALT2,
CD58-205 ENST00000526981.1 395 131aa Protein coding - H0YDI1 - CDS 5' and 3' incomplete, TSL:1,
CD58-204 ENST00000464088.5 874 237aa Nonsense mediated decay - P19256-2 - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD58
This KO Strategy loading
Red Cotton™ Notes Gene CD58 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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