CRISPR-U™ Gene Knockout Cell Line
Strategy
CD58 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD58 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CD58 |
| Gene id | 965 |
| Organism | Homo sapiens |
| Official full symbol | CD58 molecule |
| Gene type | protein-coding |
| Also known as | LFA-3, LFA3, ag3 |
| Summary | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CD58-203 | ENST00000457047.6 | 1328 | 248aa | Protein coding | CCDS44199 | P19256-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CD58-202 | ENST00000369489.10 | 1086 | 250aa | Protein coding | CCDS888 | P19256-1 | NM_001779.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CD58-201 | ENST00000369487.3 | 892 | 240aa | Protein coding | - | B1AMW1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CD58-205 | ENST00000526981.1 | 395 | 131aa | Protein coding | - | H0YDI1 | - | CDS 5' and 3' incomplete, TSL:1, |
| CD58-204 | ENST00000464088.5 | 874 | 237aa | Nonsense mediated decay | - | P19256-2 | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CD58 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CD58 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: