CRISPR-U™ Gene Knockout Cell Line Strategy

PPM1F Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPM1F Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPM1F
Gene id 9647
Organism Homo sapiens
Official full symbol protein phosphatase, Mg2+/Mn2+ dependent 1F
Gene type protein-coding
Also known as CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2
Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
Genomic regions Chromosome 22
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPM1F-201 ENST00000263212.10 5149 454aa Protein coding CCDS13796 P49593-1 NM_014634.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPM1F-203 ENST00000407142.5 5587 286aa Protein coding - B5MCT7 - TSL:5, GENCODE basic,
PPM1F-202 ENST00000397495.8 1778 361aa Protein coding - A8MX49 - TSL:2, GENCODE basic,
PPM1F-205 ENST00000445205.1 650 89aa Protein coding - C9J2F3 - CDS 3' incomplete, TSL:5,
PPM1F-204 ENST00000424647.1 576 82aa Protein coding - C9JL74 - CDS 3' incomplete, TSL:2,
PPM1F-207 ENST00000486259.1 583 No protein Processed transcript - - - TSL:3,
PPM1F-208 ENST00000496143.5 544 No protein Processed transcript - - - TSL:4,
PPM1F-206 ENST00000484588.1 348 No protein Processed transcript - - - TSL:5,
PPM1F-209 ENST00000497072.1 159 No protein Processed transcript - - - TSL:3,
PPM1F-210 ENST00000623652.1 3157 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPM1F
This KO Strategy loading
Red Cotton™ Notes Gene PPM1F had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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