CRISPR-U™ Gene Knockout Cell Line
Strategy
PPM1F Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPM1F Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPM1F |
| Gene id | 9647 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase, Mg2+/Mn2+ dependent 1F |
| Gene type | protein-coding |
| Also known as | CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2 |
| Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPM1F-201 | ENST00000263212.10 | 5149 | 454aa | Protein coding | CCDS13796 | P49593-1 | NM_014634.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPM1F-203 | ENST00000407142.5 | 5587 | 286aa | Protein coding | - | B5MCT7 | - | TSL:5, GENCODE basic, |
| PPM1F-202 | ENST00000397495.8 | 1778 | 361aa | Protein coding | - | A8MX49 | - | TSL:2, GENCODE basic, |
| PPM1F-205 | ENST00000445205.1 | 650 | 89aa | Protein coding | - | C9J2F3 | - | CDS 3' incomplete, TSL:5, |
| PPM1F-204 | ENST00000424647.1 | 576 | 82aa | Protein coding | - | C9JL74 | - | CDS 3' incomplete, TSL:2, |
| PPM1F-207 | ENST00000486259.1 | 583 | No protein | Processed transcript | - | - | - | TSL:3, |
| PPM1F-208 | ENST00000496143.5 | 544 | No protein | Processed transcript | - | - | - | TSL:4, |
| PPM1F-206 | ENST00000484588.1 | 348 | No protein | Processed transcript | - | - | - | TSL:5, |
| PPM1F-209 | ENST00000497072.1 | 159 | No protein | Processed transcript | - | - | - | TSL:3, |
| PPM1F-210 | ENST00000623652.1 | 3157 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPM1F |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPM1F had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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