CRISPR-U™ Gene Knockout Cell Line Strategy

MORF4L2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MORF4L2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MORF4L2
Gene id 9643
Organism Homo sapiens
Official full symbol mortality factor 4 like 2
Gene type protein-coding
Also known as MORFL2, MRGX
Genomic regions Chromosome X
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MORF4L2-204 ENST00000433176.6 1965 288aa Protein coding CCDS14512 Q15014 - TSL:2, GENCODE basic, APPRIS P1,
MORF4L2-206 ENST00000441076.7 1866 288aa Protein coding CCDS14512 Q15014 NM_012286.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MORF4L2-208 ENST00000451301.5 1830 288aa Protein coding CCDS14512 Q15014 - TSL:2, GENCODE basic, APPRIS P1,
MORF4L2-201 ENST00000360458.5 1824 288aa Protein coding CCDS14512 Q15014 - TSL:2, GENCODE basic, APPRIS P1,
MORF4L2-205 ENST00000434230.5 899 177aa Protein coding - Q5JXX2 - CDS 3' incomplete, TSL:2,
MORF4L2-207 ENST00000442614.5 869 158aa Protein coding - Q5JXX1 - CDS 3' incomplete, TSL:2,
MORF4L2-202 ENST00000418819.5 849 177aa Protein coding - Q5JXX2 - CDS 3' incomplete, TSL:3,
MORF4L2-203 ENST00000422355.5 562 67aa Protein coding - Q5JXX6 - CDS 3' incomplete, TSL:2,
MORF4L2-212 ENST00000492116.5 582 No protein Processed transcript - - - TSL:2,
MORF4L2-210 ENST00000474653.5 573 No protein Processed transcript - - - TSL:2,
MORF4L2-213 ENST00000498064.1 451 No protein Processed transcript - - - TSL:2,
MORF4L2-209 ENST00000467755.5 370 No protein Processed transcript - - - TSL:1,
MORF4L2-211 ENST00000488331.1 326 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MORF4L2
This KO Strategy loading
Red Cotton™ Notes Gene MORF4L2 had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
Ubigene Red Cotton Workflow

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