CRISPR-U™ Gene Knockout Cell Line Strategy

ISG15 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ISG15 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ISG15
Gene id 9636
Organism Homo sapiens
Official full symbol ISG15 ubiquitin like modifier
Gene type protein-coding
Also known as G1P2, IFI15, IMD38, IP17, UCRP, hUCRP
Summary The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ISG15-203 ENST00000649529.1 637 165aa Protein coding CCDS6 P05161 NM_005101.4 GENCODE basic, APPRIS P1, MANE Select v0.92,
ISG15-202 ENST00000624697.4 787 157aa Protein coding - A0A096LPJ4 - TSL:3, GENCODE basic,
ISG15-201 ENST00000624652.1 657 143aa Protein coding - A0A096LNZ9 - CDS 3' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ISG15
This KO Strategy loading
Red Cotton™ Notes Gene ISG15 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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