CRISPR-U™ Gene Knockout Cell Line Strategy

GDA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GDA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GDA
Gene id 9615
Organism Homo sapiens
Official full symbol guanine deaminase
Gene type protein-coding
Also known as CYPIN, GAH, GUANASE, NEDASIN
Summary This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GDA-202 ENST00000358399.8 5367 454aa Protein coding CCDS6641 Q9Y2T3-1 NM_004293.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GDA-209 ENST00000545168.5 5362 380aa Protein coding CCDS56577 Q9Y2T3-2 - TSL:2, GENCODE basic,
GDA-201 ENST00000238018.8 2074 471aa Protein coding CCDS56576 Q9Y2T3-3 - TSL:1, GENCODE basic,
GDA-203 ENST00000376986.5 1771 393aa Protein coding - Q5SZC6 - TSL:2, GENCODE basic,
GDA-205 ENST00000436438.1 990 179aa Protein coding - Q5SZC3 - CDS 5' incomplete, TSL:3,
GDA-204 ENST00000414671.1 694 195aa Protein coding - Q5SZC5 - CDS 3' incomplete, TSL:3,
GDA-206 ENST00000475764.5 2019 454aa Nonsense mediated decay CCDS6641 Q9Y2T3-1 - TSL:1,
GDA-208 ENST00000489618.5 888 176aa Nonsense mediated decay - H0YDZ7 - CDS 5' incomplete, TSL:3,
GDA-207 ENST00000477618.1 2359 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GDA
This KO Strategy loading
Red Cotton™ Notes Gene GDA had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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