CRISPR-U™ Gene Knockout Cell Line
Strategy
IER2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IER2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IER2 |
| Gene id | 9592 |
| Organism | Homo sapiens |
| Official full symbol | immediate early response 2 |
| Gene type | protein-coding |
| Also known as | ETR101 |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IER2-203 | ENST00000588173.1 | 2934 | 223aa | Protein coding | CCDS12295 | A0A024R7H1 Q9BTL4 | - | TSL:NA, GENCODE basic, APPRIS P1, |
| IER2-201 | ENST00000292433.4 | 2110 | 223aa | Protein coding | CCDS12295 | A0A024R7H1 Q9BTL4 | NM_004907.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IER2-202 | ENST00000587885.1 | 1291 | 223aa | Protein coding | CCDS12295 | A0A024R7H1 Q9BTL4 | - | TSL:3, GENCODE basic, APPRIS P1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IER2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IER2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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