CRISPR-U™ Gene Knockout Cell Line Strategy

IER2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IER2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IER2
Gene id 9592
Organism Homo sapiens
Official full symbol immediate early response 2
Gene type protein-coding
Also known as ETR101
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IER2-203 ENST00000588173.1 2934 223aa Protein coding CCDS12295 A0A024R7H1 Q9BTL4 - TSL:NA, GENCODE basic, APPRIS P1,
IER2-201 ENST00000292433.4 2110 223aa Protein coding CCDS12295 A0A024R7H1 Q9BTL4 NM_004907.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IER2-202 ENST00000587885.1 1291 223aa Protein coding CCDS12295 A0A024R7H1 Q9BTL4 - TSL:3, GENCODE basic, APPRIS P1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IER2
This KO Strategy loading
Red Cotton™ Notes Gene IER2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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