CRISPR-U™ Gene Knockout Cell Line
Strategy
BAG2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BAG2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BAG2 |
| Gene id | 9532 |
| Organism | Homo sapiens |
| Official full symbol | BAG cochaperone 2 |
| Gene type | protein-coding |
| Also known as | BAG-2, dJ417I1.2 |
| Summary | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BAG2-201 | ENST00000370693.5 | 6651 | 211aa | Protein coding | CCDS4961 | O95816-1 | NM_004282.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BAG2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BAG2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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