CRISPR-U™ Gene Knockout Cell Line Strategy

MPDU1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MPDU1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MPDU1
Gene id 9526
Organism Homo sapiens
Official full symbol mannose-P-dolichol utilization defect 1
Gene type protein-coding
Also known as CDGIF, HBEBP2BPA, Lec35, My008, PP3958, PQLC5, SL15, SLC66A5
Summary This gene encodes an endoplasmic reticulum membrane protein that is required for utilization of the mannose donor mannose-P-dolichol in the synthesis of lipid-linked oligosaccharides and glycosylphosphatidylinositols. Mutations in this gene result in congenital disorder of glycosylation type If. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 17
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MPDU1-201 ENST00000250124.11 1416 247aa Protein coding CCDS11115 O75352-1 NM_004870.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MPDU1-203 ENST00000396501.8 1125 192aa Protein coding CCDS82054 J3QW43 - TSL:2, GENCODE basic,
MPDU1-204 ENST00000423172.6 888 186aa Protein coding - O75352-2 - TSL:2, GENCODE basic,
MPDU1-223 ENST00000582151.1 728 52aa Protein coding - J3KSI4 - TSL:NA, GENCODE basic,
MPDU1-218 ENST00000579445.5 582 193aa Protein coding - J3QRD5 - CDS 3' incomplete, TSL:3,
MPDU1-224 ENST00000584378.5 475 154aa Protein coding - J3KT75 - CDS 3' incomplete, TSL:4,
MPDU1-221 ENST00000581380.1 456 152aa Protein coding - J3QQZ4 - CDS 5' and 3' incomplete, TSL:3,
MPDU1-227 ENST00000585217.5 438 116aa Protein coding - J3KTK8 - CDS 5' incomplete, TSL:3,
MPDU1-211 ENST00000572936.5 1102 61aa Nonsense mediated decay - I3L1D2 - TSL:5,
MPDU1-207 ENST00000571822.5 1020 61aa Nonsense mediated decay - I3L1D2 - TSL:2,
MPDU1-209 ENST00000572719.5 917 109aa Nonsense mediated decay - I3L295 - TSL:5,
MPDU1-212 ENST00000574558.1 900 32aa Nonsense mediated decay - I3L4T1 - CDS 5' incomplete, TSL:3,
MPDU1-202 ENST00000359822.10 840 61aa Nonsense mediated decay - I3L1D2 - TSL:3,
MPDU1-215 ENST00000576272.5 809 54aa Nonsense mediated decay - I3L405 - CDS 5' incomplete, TSL:2,
MPDU1-220 ENST00000580834.5 714 109aa Nonsense mediated decay - I3L295 - TSL:5,
MPDU1-205 ENST00000570458.5 591 61aa Nonsense mediated decay - I3L261 - CDS 5' incomplete, TSL:3,
MPDU1-225 ENST00000584479.5 572 109aa Nonsense mediated decay - I3L295 - TSL:2,
MPDU1-219 ENST00000580708.5 563 101aa Nonsense mediated decay - J3QS48 - TSL:5,
MPDU1-214 ENST00000576066.5 491 75aa Nonsense mediated decay - I3L4E0 - TSL:3,
MPDU1-216 ENST00000577088.5 1645 No protein Retained intron - - - TSL:2,
MPDU1-210 ENST00000572836.5 1523 No protein Retained intron - - - TSL:2,
MPDU1-208 ENST00000571877.1 1195 No protein Retained intron - - - TSL:1,
MPDU1-222 ENST00000581886.5 924 No protein Retained intron - - - TSL:3,
MPDU1-226 ENST00000585188.5 863 No protein Retained intron - - - TSL:3,
MPDU1-206 ENST00000571391.5 677 No protein Retained intron - - - TSL:2,
MPDU1-217 ENST00000578267.5 582 No protein Retained intron - - - TSL:3,
MPDU1-213 ENST00000575256.1 392 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MPDU1
This KO Strategy loading
Red Cotton™ Notes Gene MPDU1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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