CRISPR-U™ Gene Knockout Cell Line Strategy

MAPK8IP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAPK8IP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAPK8IP1
Gene id 9479
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase 8 interacting protein 1
Gene type protein-coding
Also known as IB1, JIP-1, JIP1, PRKM8IP
Summary This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAPK8IP1-201 ENST00000241014.6 3050 711aa Protein coding CCDS7916 Q9UQF2 NM_005456.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
MAPK8IP1-202 ENST00000395629.2 3180 701aa Protein coding - E9PBB9 - TSL:5, GENCODE basic, APPRIS ALT2,
MAPK8IP1-203 ENST00000497090.1 501 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAPK8IP1
This KO Strategy loading
Red Cotton™ Notes Gene MAPK8IP1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×