CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPK8IP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPK8IP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPK8IP1 |
| Gene id | 9479 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase 8 interacting protein 1 |
| Gene type | protein-coding |
| Also known as | IB1, JIP-1, JIP1, PRKM8IP |
| Summary | This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPK8IP1-201 | ENST00000241014.6 | 3050 | 711aa | Protein coding | CCDS7916 | Q9UQF2 | NM_005456.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| MAPK8IP1-202 | ENST00000395629.2 | 3180 | 701aa | Protein coding | - | E9PBB9 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| MAPK8IP1-203 | ENST00000497090.1 | 501 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPK8IP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPK8IP1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: