CRISPR-U™ Gene Knockout Cell Line Strategy

GSTO1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GSTO1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GSTO1
Gene id 9446
Organism Homo sapiens
Official full symbol glutathione S-transferase omega 1
Gene type protein-coding
Also known as GSTO 1-1, GSTTLp28, HEL-S-21, P28, SPG-R
Summary The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 10
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GSTO1-207 ENST00000539281.5 1052 213aa Protein coding CCDS53573 P78417-3 - TSL:5, GENCODE basic,
GSTO1-201 ENST00000369710.8 900 208aa Protein coding CCDS53572 P78417-2 - TSL:2, GENCODE basic,
GSTO1-202 ENST00000369713.10 813 241aa Protein coding CCDS7555 P78417-1 NM_004832.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GSTO1-204 ENST00000445155.5 829 200aa Protein coding - Q5TA02 - CDS 3' incomplete, TSL:2,
GSTO1-203 ENST00000432659.1 592 180aa Protein coding - Q5TA01 - CDS 3' incomplete, TSL:2,
GSTO1-206 ENST00000493946.1 874 No protein Processed transcript - - - TSL:2,
GSTO1-205 ENST00000470554.5 547 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GSTO1
This KO Strategy loading
Red Cotton™ Notes Gene GSTO1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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