CRISPR-U™ Gene Knockout Cell Line
Strategy
GSTO1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GSTO1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GSTO1 |
| Gene id | 9446 |
| Organism | Homo sapiens |
| Official full symbol | glutathione S-transferase omega 1 |
| Gene type | protein-coding |
| Also known as | GSTO 1-1, GSTTLp28, HEL-S-21, P28, SPG-R |
| Summary | The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GSTO1-207 | ENST00000539281.5 | 1052 | 213aa | Protein coding | CCDS53573 | P78417-3 | - | TSL:5, GENCODE basic, |
| GSTO1-201 | ENST00000369710.8 | 900 | 208aa | Protein coding | CCDS53572 | P78417-2 | - | TSL:2, GENCODE basic, |
| GSTO1-202 | ENST00000369713.10 | 813 | 241aa | Protein coding | CCDS7555 | P78417-1 | NM_004832.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GSTO1-204 | ENST00000445155.5 | 829 | 200aa | Protein coding | - | Q5TA02 | - | CDS 3' incomplete, TSL:2, |
| GSTO1-203 | ENST00000432659.1 | 592 | 180aa | Protein coding | - | Q5TA01 | - | CDS 3' incomplete, TSL:2, |
| GSTO1-206 | ENST00000493946.1 | 874 | No protein | Processed transcript | - | - | - | TSL:2, |
| GSTO1-205 | ENST00000470554.5 | 547 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GSTO1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GSTO1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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