CRISPR-U™ Gene Knockout Cell Line
Strategy
EPSTI1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EPSTI1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EPSTI1 |
| Gene id | 94240 |
| Organism | Homo sapiens |
| Official full symbol | epithelial stromal interaction 1 |
| Gene type | protein-coding |
| Also known as | BRESI1 |
| Summary | The protein encoded by this gene has been shown to promote tumor invasion and metastasis in some invasive cancer cells when overexpressed. Expression of this gene has been shown to be upregulated by direct binding of the Kruppel like factor 8 protein to promoter sequences. The translated protein interacts with the amino terminal region of the valosin containing protein gene product, resulting in the nuclear translocation of the nuclear factor kappa B subunit 1 gene product, and activation of target genes. Overexpression of this gene has been observed in some breast cancers and in some individuals with systemic lupus erythematosus (SLE). |
| Genomic regions | Chromosome 13 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EPSTI1-202 | ENST00000313640.11 | 3160 | 410aa | Protein coding | CCDS31964 | Q96J88-3 | - | TSL:1, GENCODE basic, APPRIS P4, |
| EPSTI1-201 | ENST00000313624.12 | 3106 | 307aa | Protein coding | CCDS9387 | Q96J88-2 | NM_033255.5 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| EPSTI1-203 | ENST00000398762.7 | 957 | 318aa | Protein coding | CCDS81765 | Q96J88-1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| EPSTI1-207 | ENST00000537828.5 | 561 | 78aa | Protein coding | - | F5H799 | - | CDS 3' incomplete, TSL:4, |
| EPSTI1-208 | ENST00000538562.5 | 574 | 80aa | Nonsense mediated decay | - | F5H4H1 | - | TSL:4, |
| EPSTI1-204 | ENST00000476830.6 | 785 | No protein | Processed transcript | - | - | - | TSL:5, |
| EPSTI1-206 | ENST00000536042.1 | 598 | No protein | Processed transcript | - | - | - | TSL:4, |
| EPSTI1-205 | ENST00000535677.5 | 585 | No protein | Processed transcript | - | - | - | TSL:3, |
| EPSTI1-210 | ENST00000542706.5 | 537 | No protein | Processed transcript | - | - | - | TSL:5, |
| EPSTI1-209 | ENST00000540470.5 | 4603 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EPSTI1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EPSTI1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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