CRISPR-U™ Gene Knockout Cell Line Strategy

EPSTI1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EPSTI1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EPSTI1
Gene id 94240
Organism Homo sapiens
Official full symbol epithelial stromal interaction 1
Gene type protein-coding
Also known as BRESI1
Summary The protein encoded by this gene has been shown to promote tumor invasion and metastasis in some invasive cancer cells when overexpressed. Expression of this gene has been shown to be upregulated by direct binding of the Kruppel like factor 8 protein to promoter sequences. The translated protein interacts with the amino terminal region of the valosin containing protein gene product, resulting in the nuclear translocation of the nuclear factor kappa B subunit 1 gene product, and activation of target genes. Overexpression of this gene has been observed in some breast cancers and in some individuals with systemic lupus erythematosus (SLE).
Genomic regions Chromosome 13
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EPSTI1-202 ENST00000313640.11 3160 410aa Protein coding CCDS31964 Q96J88-3 - TSL:1, GENCODE basic, APPRIS P4,
EPSTI1-201 ENST00000313624.12 3106 307aa Protein coding CCDS9387 Q96J88-2 NM_033255.5 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
EPSTI1-203 ENST00000398762.7 957 318aa Protein coding CCDS81765 Q96J88-1 - TSL:5, GENCODE basic, APPRIS ALT2,
EPSTI1-207 ENST00000537828.5 561 78aa Protein coding - F5H799 - CDS 3' incomplete, TSL:4,
EPSTI1-208 ENST00000538562.5 574 80aa Nonsense mediated decay - F5H4H1 - TSL:4,
EPSTI1-204 ENST00000476830.6 785 No protein Processed transcript - - - TSL:5,
EPSTI1-206 ENST00000536042.1 598 No protein Processed transcript - - - TSL:4,
EPSTI1-205 ENST00000535677.5 585 No protein Processed transcript - - - TSL:3,
EPSTI1-210 ENST00000542706.5 537 No protein Processed transcript - - - TSL:5,
EPSTI1-209 ENST00000540470.5 4603 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EPSTI1
This KO Strategy loading
Red Cotton™ Notes Gene EPSTI1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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