CRISPR-U™ Gene Knockout Cell Line Strategy

GNG8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNG8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNG8
Gene id 94235
Organism Homo sapiens
Official full symbol G protein subunit gamma 8
Gene type protein-coding
Genomic regions Chromosome 19
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNG8-201 ENST00000300873.4 216 70aa Protein coding CCDS12687 Q9UK08 - TSL:1, GENCODE basic, APPRIS P1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNG8
This KO Strategy loading
Red Cotton™ Notes Gene GNG8 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
Ubigene Red Cotton Workflow

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