CRISPR-U™ Gene Knockout Cell Line Strategy

CD28 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CD28 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD28
Gene id 940
Organism Homo sapiens
Official full symbol CD28 molecule
Gene type protein-coding
Also known as Tp44
Summary The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD28-201 ENST00000324106.9 4721 220aa Protein coding CCDS2361 P10747-1 NM_006139.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CD28-202 ENST00000374481.7 4526 101aa Protein coding CCDS58749 P10747-2 - TSL:1, GENCODE basic,
CD28-203 ENST00000458610.6 705 234aa Protein coding - P10747-7 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD28
This KO Strategy loading
Red Cotton™ Notes Gene CD28 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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