CRISPR-U™ Gene Knockout Cell Line
Strategy
CD28 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD28 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CD28 |
| Gene id | 940 |
| Organism | Homo sapiens |
| Official full symbol | CD28 molecule |
| Gene type | protein-coding |
| Also known as | Tp44 |
| Summary | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CD28-201 | ENST00000324106.9 | 4721 | 220aa | Protein coding | CCDS2361 | P10747-1 | NM_006139.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CD28-202 | ENST00000374481.7 | 4526 | 101aa | Protein coding | CCDS58749 | P10747-2 | - | TSL:1, GENCODE basic, |
| CD28-203 | ENST00000458610.6 | 705 | 234aa | Protein coding | - | P10747-7 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CD28 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CD28 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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