CRISPR-U™ Gene Knockout Cell Line
Strategy
PLAA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLAA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLAA |
| Gene id | 9373 |
| Organism | Homo sapiens |
| Official full symbol | phospholipase A2 activating protein |
| Gene type | protein-coding |
| Also known as | DOA1, NDMSBA, PLA2P, PLAP |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLAA-201 | ENST00000397292.8 | 4724 | 795aa | Protein coding | CCDS35000 | Q9Y263 | NM_001031689.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PLAA-205 | ENST00000520884.5 | 2041 | 609aa | Protein coding | - | E5RIM3 | - | TSL:2, GENCODE basic, |
| PLAA-203 | ENST00000517642.5 | 868 | 290aa | Protein coding | - | H0YBW4 | - | CDS 5' and 3' incomplete, TSL:5, |
| PLAA-207 | ENST00000523212.1 | 730 | 244aa | Protein coding | - | H0YAU9 | - | CDS 5' and 3' incomplete, TSL:3, |
| PLAA-202 | ENST00000487173.5 | 625 | 136aa | Protein coding | - | H0YC16 | - | CDS 5' incomplete, TSL:2, |
| PLAA-204 | ENST00000520641.1 | 471 | No protein | Processed transcript | - | - | - | TSL:5, |
| PLAA-206 | ENST00000521968.1 | 457 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLAA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLAA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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