CRISPR-U™ Gene Knockout Cell Line Strategy

PLAA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLAA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLAA
Gene id 9373
Organism Homo sapiens
Official full symbol phospholipase A2 activating protein
Gene type protein-coding
Also known as DOA1, NDMSBA, PLA2P, PLAP
Genomic regions Chromosome 9
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLAA-201 ENST00000397292.8 4724 795aa Protein coding CCDS35000 Q9Y263 NM_001031689.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PLAA-205 ENST00000520884.5 2041 609aa Protein coding - E5RIM3 - TSL:2, GENCODE basic,
PLAA-203 ENST00000517642.5 868 290aa Protein coding - H0YBW4 - CDS 5' and 3' incomplete, TSL:5,
PLAA-207 ENST00000523212.1 730 244aa Protein coding - H0YAU9 - CDS 5' and 3' incomplete, TSL:3,
PLAA-202 ENST00000487173.5 625 136aa Protein coding - H0YC16 - CDS 5' incomplete, TSL:2,
PLAA-204 ENST00000520641.1 471 No protein Processed transcript - - - TSL:5,
PLAA-206 ENST00000521968.1 457 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLAA
This KO Strategy loading
Red Cotton™ Notes Gene PLAA had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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