CRISPR-U™ Gene Knockout Cell Line Strategy

TXNL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TXNL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TXNL1
Gene id 9352
Organism Homo sapiens
Official full symbol thioredoxin like 1
Gene type protein-coding
Also known as HEL-S-114, TRP32, TXL-1, TXNL, Txl
Genomic regions Chromosome 18
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TXNL1-201 ENST00000217515.11 6840 289aa Protein coding CCDS11961 O43396 V9HW51 NM_004786.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TXNL1-211 ENST00000590954.5 1339 289aa Protein coding CCDS11961 O43396 V9HW51 - TSL:2, GENCODE basic, APPRIS P1,
TXNL1-204 ENST00000586262.5 3541 281aa Protein coding - K7ER96 - CDS 5' incomplete, TSL:1,
TXNL1-205 ENST00000587613.1 934 138aa Protein coding - K7EML9 - CDS 3' incomplete, TSL:3,
TXNL1-208 ENST00000589329.5 896 27aa Protein coding - K7EKG2 - CDS 3' incomplete, TSL:3,
TXNL1-210 ENST00000589931.1 466 103aa Protein coding - K7EME7 - CDS 5' incomplete, TSL:3,
TXNL1-206 ENST00000587807.5 2035 69aa Nonsense mediated decay - K7EPB7 - TSL:2,
TXNL1-202 ENST00000585497.1 1647 No protein Processed transcript - - - TSL:1,
TXNL1-207 ENST00000588615.1 695 No protein Processed transcript - - - TSL:5,
TXNL1-209 ENST00000589415.1 570 No protein Processed transcript - - - TSL:5,
TXNL1-203 ENST00000586079.1 502 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TXNL1
This KO Strategy loading
Red Cotton™ Notes Gene TXNL1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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