CRISPR-U™ Gene Knockout Cell Line Strategy

SLC9A3R2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SLC9A3R2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SLC9A3R2
Gene id 9351
Organism Homo sapiens
Official full symbol SLC9A3 regulator 2
Gene type protein-coding
Also known as E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1
Summary This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SLC9A3R2-201 ENST00000424542.7 2160 337aa Protein coding CCDS45382 Q15599-1 NM_001130012.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SLC9A3R2-202 ENST00000432365.6 1564 326aa Protein coding CCDS45383 Q15599-2 - TSL:1, GENCODE basic,
SLC9A3R2-208 ENST00000566198.1 1199 226aa Protein coding CCDS58407 Q15599-3 - TSL:2, GENCODE basic,
SLC9A3R2-204 ENST00000563587.5 1037 231aa Protein coding - H3BQS0 - TSL:2, GENCODE basic,
SLC9A3R2-207 ENST00000565855.5 759 223aa Protein coding - H3BN50 - CDS 3' incomplete, TSL:2,
SLC9A3R2-203 ENST00000561844.5 738 166aa Protein coding - H3BUQ9 - CDS 5' incomplete, TSL:3,
SLC9A3R2-209 ENST00000567504.5 578 151aa Protein coding - H3BMF5 - CDS 3' incomplete, TSL:3,
SLC9A3R2-206 ENST00000565086.1 685 No protein Processed transcript - - - TSL:3,
SLC9A3R2-205 ENST00000564033.1 1087 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SLC9A3R2
This KO Strategy loading
Red Cotton™ Notes Gene SLC9A3R2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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