CRISPR-U™ Gene Knockout Cell Line
Strategy
SLC9A3R2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SLC9A3R2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SLC9A3R2 |
| Gene id | 9351 |
| Organism | Homo sapiens |
| Official full symbol | SLC9A3 regulator 2 |
| Gene type | protein-coding |
| Also known as | E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1 |
| Summary | This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SLC9A3R2-201 | ENST00000424542.7 | 2160 | 337aa | Protein coding | CCDS45382 | Q15599-1 | NM_001130012.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SLC9A3R2-202 | ENST00000432365.6 | 1564 | 326aa | Protein coding | CCDS45383 | Q15599-2 | - | TSL:1, GENCODE basic, |
| SLC9A3R2-208 | ENST00000566198.1 | 1199 | 226aa | Protein coding | CCDS58407 | Q15599-3 | - | TSL:2, GENCODE basic, |
| SLC9A3R2-204 | ENST00000563587.5 | 1037 | 231aa | Protein coding | - | H3BQS0 | - | TSL:2, GENCODE basic, |
| SLC9A3R2-207 | ENST00000565855.5 | 759 | 223aa | Protein coding | - | H3BN50 | - | CDS 3' incomplete, TSL:2, |
| SLC9A3R2-203 | ENST00000561844.5 | 738 | 166aa | Protein coding | - | H3BUQ9 | - | CDS 5' incomplete, TSL:3, |
| SLC9A3R2-209 | ENST00000567504.5 | 578 | 151aa | Protein coding | - | H3BMF5 | - | CDS 3' incomplete, TSL:3, |
| SLC9A3R2-206 | ENST00000565086.1 | 685 | No protein | Processed transcript | - | - | - | TSL:3, |
| SLC9A3R2-205 | ENST00000564033.1 | 1087 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SLC9A3R2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SLC9A3R2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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