CRISPR-U™ Gene Knockout Cell Line
Strategy
SNAP29 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SNAP29 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SNAP29 |
| Gene id | 9342 |
| Organism | Homo sapiens |
| Official full symbol | synaptosome associated protein 29 |
| Gene type | protein-coding |
| Also known as | CEDNIK, SNAP-29 |
| Summary | This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SNAP29-201 | ENST00000215730.12 | 4259 | 258aa | Protein coding | CCDS13784 | O95721 | NM_004782.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SNAP29-202 | ENST00000439214.1 | 681 | 160aa | Protein coding | - | C9JAF7 | - | CDS 3' incomplete, TSL:3, |
| SNAP29-203 | ENST00000490458.1 | 972 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SNAP29 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SNAP29 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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