CRISPR-U™ Gene Knockout Cell Line
Strategy
GLP2R Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLP2R Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLP2R |
| Gene id | 9340 |
| Organism | Homo sapiens |
| Official full symbol | glucagon like peptide 2 receptor |
| Gene type | protein-coding |
| Summary | This gene encodes a G protein-coupled receptor that is closely related to the glucagon receptor and binds to glucagon-like peptide-2 (GLP2). Signalling through GLP2 stimulates intestinal growth and increases villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLP2R-201 | ENST00000262441.10 | 4196 | 553aa | Protein coding | CCDS11150 | A0A384MTS7 O95838 | NM_004246.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GLP2R-204 | ENST00000574745.5 | 1882 | 373aa | Protein coding | - | I3L0P5 | - | TSL:2, GENCODE basic, |
| GLP2R-202 | ENST00000304773.5 | 523 | 118aa | Protein coding | - | E9PC86 | - | CDS 3' incomplete, TSL:2, |
| GLP2R-203 | ENST00000458005.2 | 2606 | 93aa | Nonsense mediated decay | - | H0Y6C2 | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GLP2R |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLP2R had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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