CRISPR-U™ Gene Knockout Cell Line Strategy

MVB12A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MVB12A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MVB12A
Gene id 93343
Organism Homo sapiens
Official full symbol multivesicular body subunit 12A
Gene type protein-coding
Also known as CFBP, FAM125A
Genomic regions Chromosome 19
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MVB12A-201 ENST00000317040.12 1250 273aa Protein coding CCDS12359 Q96EY5-1 NM_138401.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MVB12A-212 ENST00000543795.5 1154 273aa Protein coding CCDS12359 Q96EY5-1 - TSL:5, GENCODE basic, APPRIS P1,
MVB12A-211 ENST00000529939.5 1091 273aa Protein coding - E9PQA6 - TSL:3, GENCODE basic,
MVB12A-208 ENST00000528911.5 820 121aa Protein coding - E9PLL0 - CDS 3' incomplete, TSL:5,
MVB12A-214 ENST00000600514.5 795 121aa Protein coding - E9PLL0 - CDS 3' incomplete, TSL:5,
MVB12A-213 ENST00000594784.1 705 122aa Protein coding - M0QZK6 - CDS 5' incomplete, TSL:5,
MVB12A-204 ENST00000528515.5 686 199aa Protein coding - E9PJW8 - TSL:5, GENCODE basic,
MVB12A-205 ENST00000528604.5 656 133aa Protein coding - E9PLZ8 - CDS 3' incomplete, TSL:3,
MVB12A-206 ENST00000528659.5 511 No protein Processed transcript - - - TSL:2,
MVB12A-210 ENST00000529490.5 480 No protein Processed transcript - - - TSL:4,
MVB12A-203 ENST00000526234.1 934 No protein Retained intron - - - TSL:2,
MVB12A-202 ENST00000524382.1 908 No protein Retained intron - - - TSL:2,
MVB12A-209 ENST00000528997.5 786 No protein Retained intron - - - TSL:2,
MVB12A-207 ENST00000528732.1 570 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MVB12A
This KO Strategy loading
Red Cotton™ Notes Gene MVB12A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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