CRISPR-U™ Gene Knockout Cell Line
Strategy
TRIP10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRIP10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TRIP10 |
| Gene id | 9322 |
| Organism | Homo sapiens |
| Official full symbol | thyroid hormone receptor interactor 10 |
| Gene type | protein-coding |
| Also known as | CIP4, HSTP, STOT, STP, TRIP-10 |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TRIP10-201 | ENST00000313244.14 | 2207 | 601aa | Protein coding | CCDS74271 | Q15642-1 | NM_001288962.2 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| TRIP10-202 | ENST00000313285.12 | 2035 | 545aa | Protein coding | CCDS12172 | Q15642-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| TRIP10-208 | ENST00000596758.5 | 1920 | 593aa | Protein coding | CCDS74272 | W4VSQ9 | - | TSL:1, GENCODE basic, |
| TRIP10-210 | ENST00000600428.5 | 2249 | 437aa | Protein coding | - | M0R0F9 | - | TSL:5, GENCODE basic, |
| TRIP10-207 | ENST00000596673.1 | 586 | 83aa | Protein coding | - | M0R070 | - | CDS 3' incomplete, TSL:3, |
| TRIP10-212 | ENST00000600677.5 | 2022 | 343aa | Nonsense mediated decay | - | M0R2H7 | - | TSL:5, |
| TRIP10-206 | ENST00000596543.5 | 545 | No protein | Processed transcript | - | - | - | TSL:4, |
| TRIP10-213 | ENST00000601303.1 | 311 | No protein | Processed transcript | - | - | - | TSL:5, |
| TRIP10-203 | ENST00000595305.5 | 2068 | No protein | Retained intron | - | - | - | TSL:2, |
| TRIP10-211 | ENST00000600491.5 | 1493 | No protein | Retained intron | - | - | - | TSL:2, |
| TRIP10-205 | ENST00000596078.5 | 1010 | No protein | Retained intron | - | - | - | TSL:5, |
| TRIP10-204 | ENST00000595319.5 | 776 | No protein | Retained intron | - | - | - | TSL:5, |
| TRIP10-209 | ENST00000598843.1 | 469 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TRIP10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TRIP10 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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