CRISPR-U™ Gene Knockout Cell Line
Strategy
PTTG1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTTG1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTTG1 |
| Gene id | 9232 |
| Organism | Homo sapiens |
| Official full symbol | PTTG1 regulator of sister chromatid separation, securin |
| Gene type | protein-coding |
| Also known as | EAP1, HPTTG, PTTG, TUTR1 |
| Summary | The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTTG1-202 | ENST00000393964.1 | 1070 | 202aa | Protein coding | CCDS4353 | O95997 Q6IAL9 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PTTG1-205 | ENST00000520452.5 | 895 | 202aa | Protein coding | CCDS4353 | O95997 Q6IAL9 | - | TSL:3, GENCODE basic, APPRIS P1, |
| PTTG1-201 | ENST00000352433.10 | 715 | 202aa | Protein coding | CCDS4353 | O95997 Q6IAL9 | NM_004219.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PTTG1-203 | ENST00000517480.1 | 684 | 121aa | Protein coding | - | E5RJR4 | - | CDS 3' incomplete, TSL:5, |
| PTTG1-204 | ENST00000519287.1 | 670 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTTG1-206 | ENST00000523659.5 | 851 | No protein | Retained intron | - | - | - | TSL:2, |
| PTTG1-207 | ENST00000524244.5 | 434 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTTG1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTTG1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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