CRISPR-U™ Gene Knockout Cell Line Strategy

PTTG1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTTG1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTTG1
Gene id 9232
Organism Homo sapiens
Official full symbol PTTG1 regulator of sister chromatid separation, securin
Gene type protein-coding
Also known as EAP1, HPTTG, PTTG, TUTR1
Summary The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 5
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTTG1-202 ENST00000393964.1 1070 202aa Protein coding CCDS4353 O95997 Q6IAL9 - TSL:1, GENCODE basic, APPRIS P1,
PTTG1-205 ENST00000520452.5 895 202aa Protein coding CCDS4353 O95997 Q6IAL9 - TSL:3, GENCODE basic, APPRIS P1,
PTTG1-201 ENST00000352433.10 715 202aa Protein coding CCDS4353 O95997 Q6IAL9 NM_004219.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTTG1-203 ENST00000517480.1 684 121aa Protein coding - E5RJR4 - CDS 3' incomplete, TSL:5,
PTTG1-204 ENST00000519287.1 670 No protein Processed transcript - - - TSL:5,
PTTG1-206 ENST00000523659.5 851 No protein Retained intron - - - TSL:2,
PTTG1-207 ENST00000524244.5 434 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTTG1
This KO Strategy loading
Red Cotton™ Notes Gene PTTG1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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