CRISPR-U™ Gene Knockout Cell Line Strategy

ARHGEF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARHGEF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARHGEF2
Gene id 9181
Organism Homo sapiens
Official full symbol Rho/Rac guanine nucleotide exchange factor 2
Gene type protein-coding
Also known as GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40
Summary Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 1
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARHGEF2-203 ENST00000361247.9 4169 986aa Protein coding CCDS53376 Q92974-1 NM_001162383.2 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
ARHGEF2-202 ENST00000313695.11 4080 958aa Protein coding CCDS1125 Q92974-3 - TSL:1, GENCODE basic, APPRIS ALT2,
ARHGEF2-201 ENST00000313667.8 2958 985aa Protein coding CCDS53375 Q92974-2 - TSL:1, GENCODE basic, APPRIS ALT2,
ARHGEF2-220 ENST00000673475.1 4727 1129aa Protein coding - A0A5F9ZI21 - GENCODE basic, APPRIS P5,
ARHGEF2-206 ENST00000462460.6 4438 1031aa Protein coding - V9GYM8 - TSL:5, GENCODE basic, APPRIS ALT2,
ARHGEF2-208 ENST00000470541.6 1877 263aa Protein coding - V9GYF0 - CDS 5' incomplete, TSL:3,
ARHGEF2-204 ENST00000368315.8 1645 264aa Protein coding - Q5VY93 - TSL:5, GENCODE basic,
ARHGEF2-211 ENST00000471589.5 892 229aa Protein coding - V9GZ14 - CDS 3' incomplete, TSL:5,
ARHGEF2-217 ENST00000497907.5 764 214aa Protein coding - V9GYG5 - CDS 3' incomplete, TSL:3,
ARHGEF2-219 ENST00000609707.1 692 178aa Protein coding - V9GYF5 - CDS 3' incomplete, TSL:2,
ARHGEF2-216 ENST00000495070.2 571 83aa Protein coding - V9GY94 - CDS 3' incomplete, TSL:4,
ARHGEF2-205 ENST00000423422.6 420 129aa Protein coding - A0A1Y8EN19 - CDS 3' incomplete, TSL:3,
ARHGEF2-209 ENST00000470874.5 372 59aa Nonsense mediated decay - V9GZ58 - CDS 5' incomplete, TSL:3,
ARHGEF2-214 ENST00000477754.2 883 No protein Processed transcript - - - TSL:5,
ARHGEF2-212 ENST00000474428.6 742 No protein Processed transcript - - - TSL:3,
ARHGEF2-213 ENST00000476273.1 668 No protein Processed transcript - - - TSL:3,
ARHGEF2-215 ENST00000487755.1 2176 No protein Retained intron - - - TSL:2,
ARHGEF2-218 ENST00000608543.1 820 No protein Retained intron - - - TSL:3,
ARHGEF2-207 ENST00000465079.1 693 No protein Retained intron - - - TSL:2,
ARHGEF2-210 ENST00000470975.2 638 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARHGEF2
This KO Strategy loading
Red Cotton™ Notes Gene ARHGEF2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×