CRISPR-U™ Gene Knockout Cell Line
Strategy
ARHGEF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARHGEF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARHGEF2 |
| Gene id | 9181 |
| Organism | Homo sapiens |
| Official full symbol | Rho/Rac guanine nucleotide exchange factor 2 |
| Gene type | protein-coding |
| Also known as | GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40 |
| Summary | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARHGEF2-203 | ENST00000361247.9 | 4169 | 986aa | Protein coding | CCDS53376 | Q92974-1 | NM_001162383.2 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| ARHGEF2-202 | ENST00000313695.11 | 4080 | 958aa | Protein coding | CCDS1125 | Q92974-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ARHGEF2-201 | ENST00000313667.8 | 2958 | 985aa | Protein coding | CCDS53375 | Q92974-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ARHGEF2-220 | ENST00000673475.1 | 4727 | 1129aa | Protein coding | - | A0A5F9ZI21 | - | GENCODE basic, APPRIS P5, |
| ARHGEF2-206 | ENST00000462460.6 | 4438 | 1031aa | Protein coding | - | V9GYM8 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| ARHGEF2-208 | ENST00000470541.6 | 1877 | 263aa | Protein coding | - | V9GYF0 | - | CDS 5' incomplete, TSL:3, |
| ARHGEF2-204 | ENST00000368315.8 | 1645 | 264aa | Protein coding | - | Q5VY93 | - | TSL:5, GENCODE basic, |
| ARHGEF2-211 | ENST00000471589.5 | 892 | 229aa | Protein coding | - | V9GZ14 | - | CDS 3' incomplete, TSL:5, |
| ARHGEF2-217 | ENST00000497907.5 | 764 | 214aa | Protein coding | - | V9GYG5 | - | CDS 3' incomplete, TSL:3, |
| ARHGEF2-219 | ENST00000609707.1 | 692 | 178aa | Protein coding | - | V9GYF5 | - | CDS 3' incomplete, TSL:2, |
| ARHGEF2-216 | ENST00000495070.2 | 571 | 83aa | Protein coding | - | V9GY94 | - | CDS 3' incomplete, TSL:4, |
| ARHGEF2-205 | ENST00000423422.6 | 420 | 129aa | Protein coding | - | A0A1Y8EN19 | - | CDS 3' incomplete, TSL:3, |
| ARHGEF2-209 | ENST00000470874.5 | 372 | 59aa | Nonsense mediated decay | - | V9GZ58 | - | CDS 5' incomplete, TSL:3, |
| ARHGEF2-214 | ENST00000477754.2 | 883 | No protein | Processed transcript | - | - | - | TSL:5, |
| ARHGEF2-212 | ENST00000474428.6 | 742 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARHGEF2-213 | ENST00000476273.1 | 668 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARHGEF2-215 | ENST00000487755.1 | 2176 | No protein | Retained intron | - | - | - | TSL:2, |
| ARHGEF2-218 | ENST00000608543.1 | 820 | No protein | Retained intron | - | - | - | TSL:3, |
| ARHGEF2-207 | ENST00000465079.1 | 693 | No protein | Retained intron | - | - | - | TSL:2, |
| ARHGEF2-210 | ENST00000470975.2 | 638 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARHGEF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARHGEF2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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