CRISPR-U™ Gene Knockout Cell Line
Strategy
DDX60L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DDX60L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DDX60L |
| Gene id | 91351 |
| Organism | Homo sapiens |
| Official full symbol | DExD/H-box 60 like |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DDX60L-208 | ENST00000511577.5 | 6781 | 1706aa | Protein coding | - | Q5H9U9 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DDX60L-206 | ENST00000505890.5 | 4568 | 1308aa | Protein coding | - | Q5H9U9 | - | TSL:2, GENCODE basic, |
| DDX60L-205 | ENST00000505863.1 | 2605 | 868aa | Protein coding | - | H0Y8H0 | - | CDS 5' and 3' incomplete, TSL:2, |
| DDX60L-213 | ENST00000514580.5 | 813 | 195aa | Protein coding | - | H0Y939 | - | CDS 5' incomplete, TSL:5, |
| DDX60L-214 | ENST00000514748.5 | 632 | 98aa | Protein coding | - | D6RB62 | - | CDS 3' incomplete, TSL:3, |
| DDX60L-204 | ENST00000505696.5 | 588 | 124aa | Protein coding | - | D6RBA2 | - | CDS 3' incomplete, TSL:4, |
| DDX60L-209 | ENST00000512371.1 | 428 | 77aa | Protein coding | - | D6RIU1 | - | CDS 3' incomplete, TSL:3, |
| DDX60L-215 | ENST00000515088.5 | 2652 | No protein | Processed transcript | - | - | - | TSL:2, |
| DDX60L-210 | ENST00000512958.1 | 769 | No protein | Processed transcript | - | - | - | TSL:3, |
| DDX60L-203 | ENST00000505150.5 | 655 | No protein | Processed transcript | - | - | - | TSL:5, |
| DDX60L-212 | ENST00000513901.1 | 623 | No protein | Processed transcript | - | - | - | TSL:3, |
| DDX60L-202 | ENST00000504793.1 | 549 | No protein | Processed transcript | - | - | - | TSL:5, |
| DDX60L-207 | ENST00000510590.1 | 3509 | No protein | Retained intron | - | - | - | TSL:2, |
| DDX60L-201 | ENST00000503190.1 | 562 | No protein | Retained intron | - | - | - | TSL:3, |
| DDX60L-211 | ENST00000513103.1 | 507 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DDX60L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DDX60L had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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