CRISPR-U™ Gene Knockout Cell Line Strategy

DDX60L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DDX60L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DDX60L
Gene id 91351
Organism Homo sapiens
Official full symbol DExD/H-box 60 like
Gene type protein-coding
Summary This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 4
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DDX60L-208 ENST00000511577.5 6781 1706aa Protein coding - Q5H9U9 - TSL:5, GENCODE basic, APPRIS P1,
DDX60L-206 ENST00000505890.5 4568 1308aa Protein coding - Q5H9U9 - TSL:2, GENCODE basic,
DDX60L-205 ENST00000505863.1 2605 868aa Protein coding - H0Y8H0 - CDS 5' and 3' incomplete, TSL:2,
DDX60L-213 ENST00000514580.5 813 195aa Protein coding - H0Y939 - CDS 5' incomplete, TSL:5,
DDX60L-214 ENST00000514748.5 632 98aa Protein coding - D6RB62 - CDS 3' incomplete, TSL:3,
DDX60L-204 ENST00000505696.5 588 124aa Protein coding - D6RBA2 - CDS 3' incomplete, TSL:4,
DDX60L-209 ENST00000512371.1 428 77aa Protein coding - D6RIU1 - CDS 3' incomplete, TSL:3,
DDX60L-215 ENST00000515088.5 2652 No protein Processed transcript - - - TSL:2,
DDX60L-210 ENST00000512958.1 769 No protein Processed transcript - - - TSL:3,
DDX60L-203 ENST00000505150.5 655 No protein Processed transcript - - - TSL:5,
DDX60L-212 ENST00000513901.1 623 No protein Processed transcript - - - TSL:3,
DDX60L-202 ENST00000504793.1 549 No protein Processed transcript - - - TSL:5,
DDX60L-207 ENST00000510590.1 3509 No protein Retained intron - - - TSL:2,
DDX60L-201 ENST00000503190.1 562 No protein Retained intron - - - TSL:3,
DDX60L-211 ENST00000513103.1 507 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DDX60L
This KO Strategy loading
Red Cotton™ Notes Gene DDX60L had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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