CRISPR-U™ Gene Knockout Cell Line
Strategy
AIFM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AIFM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AIFM1 |
| Gene id | 9131 |
| Organism | Homo sapiens |
| Official full symbol | apoptosis inducing factor mitochondria associated 1 |
| Gene type | protein-coding |
| Also known as | AIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL |
| Summary | This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AIFM1-229 | ENST00000676229.1 | 4157 | 609aa | Protein coding | CCDS14619 | - | - | GENCODE basic, APPRIS P1, |
| AIFM1-209 | ENST00000535724.6 | 2248 | 324aa | Protein coding | CCDS48167 | O95831-4 | - | TSL:1, GENCODE basic, |
| AIFM1-201 | ENST00000287295.8 | 2222 | 613aa | Protein coding | CCDS14618 | O95831-1 | NM_004208.4 | TSL:1, GENCODE basic, MANE Select v0.92, |
| AIFM1-205 | ENST00000460436.6 | 1925 | 274aa | Protein coding | CCDS48166 | E9PMA0 | - | TSL:1, GENCODE basic, |
| AIFM1-210 | ENST00000674546.1 | 2233 | 589aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-202 | ENST00000319908.8 | 2192 | 611aa | Protein coding | - | O95831-3 | - | TSL:1, GENCODE basic, |
| AIFM1-221 | ENST00000675092.1 | 2154 | 622aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-224 | ENST00000675427.1 | 2123 | 612aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-226 | ENST00000675857.1 | 2113 | 611aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-223 | ENST00000675240.1 | 2107 | 565aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-230 | ENST00000676328.1 | 2086 | 612aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-231 | ENST00000676436.1 | 2048 | 609aa | Protein coding | - | - | - | GENCODE basic, |
| AIFM1-203 | ENST00000346424.6 | 1200 | 326aa | Protein coding | - | O95831-2 | - | TSL:2, GENCODE basic, |
| AIFM1-214 | ENST00000674601.1 | 711 | 229aa | Protein coding | - | - | - | CDS 5' incomplete, |
| AIFM1-220 | ENST00000675050.1 | 3083 | 609aa | Nonsense mediated decay | CCDS14619 | - | - | - |
| AIFM1-216 | ENST00000674957.1 | 2434 | 293aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| AIFM1-206 | ENST00000527892.5 | 2105 | 43aa | Nonsense mediated decay | - | E9PRR0 | - | TSL:2, |
| AIFM1-211 | ENST00000674555.1 | 2077 | 98aa | Nonsense mediated decay | - | - | - | - |
| AIFM1-204 | ENST00000416073.7 | 2061 | 441aa | Nonsense mediated decay | - | O95831-4 | - | TSL:1, |
| AIFM1-219 | ENST00000675037.1 | 1962 | 460aa | Nonsense mediated decay | - | - | - | - |
| AIFM1-215 | ENST00000674722.1 | 1905 | 248aa | Nonsense mediated decay | - | - | - | - |
| AIFM1-217 | ENST00000674997.1 | 1883 | 43aa | Nonsense mediated decay | - | - | - | - |
| AIFM1-225 | ENST00000675774.1 | 1454 | 43aa | Nonsense mediated decay | - | - | - | - |
| AIFM1-228 | ENST00000676144.1 | 917 | 61aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| AIFM1-207 | ENST00000529877.1 | 723 | 89aa | Nonsense mediated decay | - | E9PMQ8 | - | TSL:3, |
| AIFM1-227 | ENST00000676048.1 | 5137 | No protein | Retained intron | - | - | - | - |
| AIFM1-212 | ENST00000674582.1 | 2200 | No protein | Retained intron | - | - | - | - |
| AIFM1-222 | ENST00000675111.1 | 1920 | No protein | Retained intron | - | - | - | - |
| AIFM1-218 | ENST00000675015.1 | 1689 | No protein | Retained intron | - | - | - | - |
| AIFM1-213 | ENST00000674591.1 | 1485 | No protein | Retained intron | - | - | - | - |
| AIFM1-208 | ENST00000533719.2 | 1465 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AIFM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AIFM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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