CRISPR-U™ Gene Knockout Cell Line Strategy

AIFM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AIFM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AIFM1
Gene id 9131
Organism Homo sapiens
Official full symbol apoptosis inducing factor mitochondria associated 1
Gene type protein-coding
Summary This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Genomic regions Chromosome X
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AIFM1-229 ENST00000676229.1 4157 609aa Protein coding CCDS14619 - - GENCODE basic, APPRIS P1,
AIFM1-209 ENST00000535724.6 2248 324aa Protein coding CCDS48167 O95831-4 - TSL:1, GENCODE basic,
AIFM1-201 ENST00000287295.8 2222 613aa Protein coding CCDS14618 O95831-1 NM_004208.4 TSL:1, GENCODE basic, MANE Select v0.92,
AIFM1-205 ENST00000460436.6 1925 274aa Protein coding CCDS48166 E9PMA0 - TSL:1, GENCODE basic,
AIFM1-210 ENST00000674546.1 2233 589aa Protein coding - - - GENCODE basic,
AIFM1-202 ENST00000319908.8 2192 611aa Protein coding - O95831-3 - TSL:1, GENCODE basic,
AIFM1-221 ENST00000675092.1 2154 622aa Protein coding - - - GENCODE basic,
AIFM1-224 ENST00000675427.1 2123 612aa Protein coding - - - GENCODE basic,
AIFM1-226 ENST00000675857.1 2113 611aa Protein coding - - - GENCODE basic,
AIFM1-223 ENST00000675240.1 2107 565aa Protein coding - - - GENCODE basic,
AIFM1-230 ENST00000676328.1 2086 612aa Protein coding - - - GENCODE basic,
AIFM1-231 ENST00000676436.1 2048 609aa Protein coding - - - GENCODE basic,
AIFM1-203 ENST00000346424.6 1200 326aa Protein coding - O95831-2 - TSL:2, GENCODE basic,
AIFM1-214 ENST00000674601.1 711 229aa Protein coding - - - CDS 5' incomplete,
AIFM1-220 ENST00000675050.1 3083 609aa Nonsense mediated decay CCDS14619 - - -
AIFM1-216 ENST00000674957.1 2434 293aa Nonsense mediated decay - - - CDS 5' incomplete,
AIFM1-206 ENST00000527892.5 2105 43aa Nonsense mediated decay - E9PRR0 - TSL:2,
AIFM1-211 ENST00000674555.1 2077 98aa Nonsense mediated decay - - - -
AIFM1-204 ENST00000416073.7 2061 441aa Nonsense mediated decay - O95831-4 - TSL:1,
AIFM1-219 ENST00000675037.1 1962 460aa Nonsense mediated decay - - - -
AIFM1-215 ENST00000674722.1 1905 248aa Nonsense mediated decay - - - -
AIFM1-217 ENST00000674997.1 1883 43aa Nonsense mediated decay - - - -
AIFM1-225 ENST00000675774.1 1454 43aa Nonsense mediated decay - - - -
AIFM1-228 ENST00000676144.1 917 61aa Nonsense mediated decay - - - CDS 5' incomplete,
AIFM1-207 ENST00000529877.1 723 89aa Nonsense mediated decay - E9PMQ8 - TSL:3,
AIFM1-227 ENST00000676048.1 5137 No protein Retained intron - - - -
AIFM1-212 ENST00000674582.1 2200 No protein Retained intron - - - -
AIFM1-222 ENST00000675111.1 1920 No protein Retained intron - - - -
AIFM1-218 ENST00000675015.1 1689 No protein Retained intron - - - -
AIFM1-213 ENST00000674591.1 1485 No protein Retained intron - - - -
AIFM1-208 ENST00000533719.2 1465 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AIFM1
This KO Strategy loading
Red Cotton™ Notes Gene AIFM1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×