CRISPR-U™ Gene Knockout Cell Line
Strategy
LMF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LMF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LMF2 |
| Gene id | 91289 |
| Organism | Homo sapiens |
| Official full symbol | lipase maturation factor 2 |
| Gene type | protein-coding |
| Also known as | TMEM112B, TMEM153 |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LMF2-201 | ENST00000216080.5 | 2658 | 682aa | Protein coding | CCDS87035 | Q9BU23-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| LMF2-202 | ENST00000474879.7 | 2593 | 707aa | Protein coding | CCDS14093 | Q9BU23-1 | NM_033200.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| LMF2-204 | ENST00000505981.5 | 824 | No protein | Processed transcript | - | - | - | TSL:5, |
| LMF2-203 | ENST00000504717.1 | 1233 | No protein | Retained intron | - | - | - | TSL:5, |
| LMF2-206 | ENST00000514938.1 | 960 | No protein | Retained intron | - | - | - | TSL:5, |
| LMF2-205 | ENST00000507607.1 | 605 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LMF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LMF2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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