CRISPR-U™ Gene Knockout Cell Line Strategy

LMF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LMF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LMF2
Gene id 91289
Organism Homo sapiens
Official full symbol lipase maturation factor 2
Gene type protein-coding
Also known as TMEM112B, TMEM153
Genomic regions Chromosome 22
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LMF2-201 ENST00000216080.5 2658 682aa Protein coding CCDS87035 Q9BU23-2 - TSL:2, GENCODE basic, APPRIS ALT2,
LMF2-202 ENST00000474879.7 2593 707aa Protein coding CCDS14093 Q9BU23-1 NM_033200.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
LMF2-204 ENST00000505981.5 824 No protein Processed transcript - - - TSL:5,
LMF2-203 ENST00000504717.1 1233 No protein Retained intron - - - TSL:5,
LMF2-206 ENST00000514938.1 960 No protein Retained intron - - - TSL:5,
LMF2-205 ENST00000507607.1 605 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LMF2
This KO Strategy loading
Red Cotton™ Notes Gene LMF2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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