CRISPR-U™ Gene Knockout Cell Line Strategy

LATS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LATS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LATS1
Gene id 9113
Organism Homo sapiens
Official full symbol large tumor suppressor kinase 1
Gene type protein-coding
Also known as WARTS, wts
Summary The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Genomic regions Chromosome 6
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LATS1-207 ENST00000543571.6 7362 1130aa Protein coding CCDS34551 O95835-1 NM_004690.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LATS1-201 ENST00000253339.9 4256 1130aa Protein coding CCDS34551 O95835-1 - TSL:1, GENCODE basic, APPRIS P1,
LATS1-202 ENST00000392273.7 2567 690aa Protein coding CCDS59040 O95835-2 - TSL:1, GENCODE basic,
LATS1-204 ENST00000458696.2 602 200aa Protein coding - H0YG09 - CDS 5' and 3' incomplete, TSL:3,
LATS1-203 ENST00000441107.5 3879 135aa Nonsense mediated decay - Q6PJG3 - TSL:1,
LATS1-205 ENST00000542720.1 1096 135aa Nonsense mediated decay - Q6PJG3 - TSL:1,
LATS1-206 ENST00000542747.5 3284 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LATS1
This KO Strategy loading
Red Cotton™ Notes Gene LATS1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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