CRISPR-U™ Gene Knockout Cell Line
Strategy
LATS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LATS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LATS1 |
| Gene id | 9113 |
| Organism | Homo sapiens |
| Official full symbol | large tumor suppressor kinase 1 |
| Gene type | protein-coding |
| Also known as | WARTS, wts |
| Summary | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LATS1-207 | ENST00000543571.6 | 7362 | 1130aa | Protein coding | CCDS34551 | O95835-1 | NM_004690.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LATS1-201 | ENST00000253339.9 | 4256 | 1130aa | Protein coding | CCDS34551 | O95835-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| LATS1-202 | ENST00000392273.7 | 2567 | 690aa | Protein coding | CCDS59040 | O95835-2 | - | TSL:1, GENCODE basic, |
| LATS1-204 | ENST00000458696.2 | 602 | 200aa | Protein coding | - | H0YG09 | - | CDS 5' and 3' incomplete, TSL:3, |
| LATS1-203 | ENST00000441107.5 | 3879 | 135aa | Nonsense mediated decay | - | Q6PJG3 | - | TSL:1, |
| LATS1-205 | ENST00000542720.1 | 1096 | 135aa | Nonsense mediated decay | - | Q6PJG3 | - | TSL:1, |
| LATS1-206 | ENST00000542747.5 | 3284 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LATS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LATS1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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