CRISPR-U™ Gene Knockout Cell Line
Strategy
NMI Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NMI Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NMI |
| Gene id | 9111 |
| Organism | Homo sapiens |
| Official full symbol | N-myc and STAT interactor |
| Gene type | protein-coding |
| Summary | NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NMI-201 | ENST00000243346.10 | 1229 | 307aa | Protein coding | CCDS2192 | Q13287 | NM_004688.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NMI-202 | ENST00000414946.1 | 581 | 28aa | Protein coding | - | C9JW17 | - | CDS 3' incomplete, TSL:5, |
| NMI-204 | ENST00000491771.5 | 2094 | No protein | Retained intron | - | - | - | TSL:2, |
| NMI-203 | ENST00000477072.1 | 455 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NMI |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NMI had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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