CRISPR-U™ Gene Knockout Cell Line Strategy

NMI Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NMI Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NMI
Gene id 9111
Organism Homo sapiens
Official full symbol N-myc and STAT interactor
Gene type protein-coding
Summary NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
Genomic regions Chromosome 2
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NMI-201 ENST00000243346.10 1229 307aa Protein coding CCDS2192 Q13287 NM_004688.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NMI-202 ENST00000414946.1 581 28aa Protein coding - C9JW17 - CDS 3' incomplete, TSL:5,
NMI-204 ENST00000491771.5 2094 No protein Retained intron - - - TSL:2,
NMI-203 ENST00000477072.1 455 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NMI
This KO Strategy loading
Red Cotton™ Notes Gene NMI had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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