CRISPR-U™ Gene Knockout Cell Line Strategy

KNSTRN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KNSTRN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KNSTRN
Gene id 90417
Organism Homo sapiens
Official full symbol kinetochore localized astrin (SPAG5) binding protein
Gene type protein-coding
Also known as C15orf23, HSD11, SKAP, TRAF4AF1
Genomic regions Chromosome 15
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KNSTRN-202 ENST00000416151.6 1899 286aa Protein coding CCDS45226 Q9Y448-2 - TSL:3, GENCODE basic, APPRIS ALT2,
KNSTRN-201 ENST00000249776.12 1714 316aa Protein coding CCDS42021 Q9Y448-1 - TSL:1, GENCODE basic, APPRIS P2,
KNSTRN-203 ENST00000448395.6 1464 241aa Protein coding CCDS45227 Q9Y448-3 - TSL:2, GENCODE basic, APPRIS ALT2,
KNSTRN-216 ENST00000608100.5 1495 238aa Protein coding - V9GY01 - TSL:1, GENCODE basic, APPRIS ALT2,
KNSTRN-204 ENST00000557920.1 651 217aa Protein coding - H0YM11 - CDS 5' and 3' incomplete, TSL:3,
KNSTRN-212 ENST00000560981.5 1598 126aa Nonsense mediated decay - H0YLH8 - TSL:2,
KNSTRN-213 ENST00000561169.5 1388 124aa Nonsense mediated decay - H0YKF8 - CDS 5' incomplete, TSL:2,
KNSTRN-210 ENST00000560321.5 940 128aa Nonsense mediated decay - H0YLA7 - CDS 5' incomplete, TSL:3,
KNSTRN-209 ENST00000560220.5 903 67aa Nonsense mediated decay - H0YNN6 - CDS 5' incomplete, TSL:5,
KNSTRN-206 ENST00000559304.5 664 64aa Nonsense mediated decay - H0YKD4 - CDS 5' incomplete, TSL:3,
KNSTRN-205 ENST00000559083.1 405 27aa Nonsense mediated decay - H0YN50 - CDS 5' incomplete, TSL:3,
KNSTRN-207 ENST00000559591.5 665 No protein Retained intron - - - TSL:2,
KNSTRN-215 ENST00000561367.1 630 No protein Retained intron - - - TSL:2,
KNSTRN-211 ENST00000560673.1 623 No protein Retained intron - - - TSL:2,
KNSTRN-208 ENST00000559604.1 535 No protein Retained intron - - - TSL:4,
KNSTRN-214 ENST00000561196.5 534 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KNSTRN
This KO Strategy loading
Red Cotton™ Notes Gene KNSTRN had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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