CRISPR-U™ Gene Knockout Cell Line
Strategy
KNSTRN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KNSTRN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KNSTRN |
| Gene id | 90417 |
| Organism | Homo sapiens |
| Official full symbol | kinetochore localized astrin (SPAG5) binding protein |
| Gene type | protein-coding |
| Also known as | C15orf23, HSD11, SKAP, TRAF4AF1 |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KNSTRN-202 | ENST00000416151.6 | 1899 | 286aa | Protein coding | CCDS45226 | Q9Y448-2 | - | TSL:3, GENCODE basic, APPRIS ALT2, |
| KNSTRN-201 | ENST00000249776.12 | 1714 | 316aa | Protein coding | CCDS42021 | Q9Y448-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| KNSTRN-203 | ENST00000448395.6 | 1464 | 241aa | Protein coding | CCDS45227 | Q9Y448-3 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| KNSTRN-216 | ENST00000608100.5 | 1495 | 238aa | Protein coding | - | V9GY01 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| KNSTRN-204 | ENST00000557920.1 | 651 | 217aa | Protein coding | - | H0YM11 | - | CDS 5' and 3' incomplete, TSL:3, |
| KNSTRN-212 | ENST00000560981.5 | 1598 | 126aa | Nonsense mediated decay | - | H0YLH8 | - | TSL:2, |
| KNSTRN-213 | ENST00000561169.5 | 1388 | 124aa | Nonsense mediated decay | - | H0YKF8 | - | CDS 5' incomplete, TSL:2, |
| KNSTRN-210 | ENST00000560321.5 | 940 | 128aa | Nonsense mediated decay | - | H0YLA7 | - | CDS 5' incomplete, TSL:3, |
| KNSTRN-209 | ENST00000560220.5 | 903 | 67aa | Nonsense mediated decay | - | H0YNN6 | - | CDS 5' incomplete, TSL:5, |
| KNSTRN-206 | ENST00000559304.5 | 664 | 64aa | Nonsense mediated decay | - | H0YKD4 | - | CDS 5' incomplete, TSL:3, |
| KNSTRN-205 | ENST00000559083.1 | 405 | 27aa | Nonsense mediated decay | - | H0YN50 | - | CDS 5' incomplete, TSL:3, |
| KNSTRN-207 | ENST00000559591.5 | 665 | No protein | Retained intron | - | - | - | TSL:2, |
| KNSTRN-215 | ENST00000561367.1 | 630 | No protein | Retained intron | - | - | - | TSL:2, |
| KNSTRN-211 | ENST00000560673.1 | 623 | No protein | Retained intron | - | - | - | TSL:2, |
| KNSTRN-208 | ENST00000559604.1 | 535 | No protein | Retained intron | - | - | - | TSL:4, |
| KNSTRN-214 | ENST00000561196.5 | 534 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KNSTRN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KNSTRN had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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