CRISPR-U™ Gene Knockout Cell Line
Strategy
BAZ1B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BAZ1B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BAZ1B |
| Gene id | 9031 |
| Organism | Homo sapiens |
| Official full symbol | bromodomain adjacent to zinc finger domain 1B |
| Gene type | protein-coding |
| Also known as | WBSCR10, WBSCR9, WSTF |
| Summary | This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BAZ1B-201 | ENST00000339594.9 | 6115 | 1483aa | Protein coding | CCDS5549 | Q9UIG0-1 | NM_032408.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BAZ1B-202 | ENST00000404251.1 | 5428 | 1483aa | Protein coding | CCDS5549 | Q9UIG0-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| BAZ1B-203 | ENST00000466844.1 | 827 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BAZ1B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BAZ1B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: