CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP3K14 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP3K14 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP3K14 |
| Gene id | 9020 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase kinase kinase 14 |
| Gene type | protein-coding |
| Also known as | FTDCR1B, HS, HSNIK, NIK |
| Summary | This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP3K14-202 | ENST00000376926.8 | 4583 | 947aa | Protein coding | CCDS74079 | Q99558 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MAP3K14-201 | ENST00000344686.8 | 4442 | 947aa | Protein coding | CCDS74079 | Q99558 | NM_003954.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAP3K14-205 | ENST00000617331.3 | 3087 | 947aa | Protein coding | CCDS74079 | A0A087WXF1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MAP3K14-206 | ENST00000680632.1 | 3406 | 266aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| MAP3K14-203 | ENST00000586644.2 | 3266 | No protein | Retained intron | - | - | - | TSL:4, |
| MAP3K14-204 | ENST00000592267.1 | 3152 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP3K14 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP3K14 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: