CRISPR-U™ Gene Knockout Cell Line Strategy

MAP3K14 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP3K14 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP3K14
Gene id 9020
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase kinase kinase 14
Gene type protein-coding
Also known as FTDCR1B, HS, HSNIK, NIK
Summary This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
Genomic regions Chromosome 17
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP3K14-202 ENST00000376926.8 4583 947aa Protein coding CCDS74079 Q99558 - TSL:1, GENCODE basic, APPRIS P1,
MAP3K14-201 ENST00000344686.8 4442 947aa Protein coding CCDS74079 Q99558 NM_003954.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAP3K14-205 ENST00000617331.3 3087 947aa Protein coding CCDS74079 A0A087WXF1 - TSL:5, GENCODE basic, APPRIS P1,
MAP3K14-206 ENST00000680632.1 3406 266aa Nonsense mediated decay - - - CDS 5' incomplete,
MAP3K14-203 ENST00000586644.2 3266 No protein Retained intron - - - TSL:4,
MAP3K14-204 ENST00000592267.1 3152 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP3K14
This KO Strategy loading
Red Cotton™ Notes Gene MAP3K14 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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