CRISPR-U™ Gene Knockout Cell Line
Strategy
MPZL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPZL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MPZL1 |
| Gene id | 9019 |
| Organism | Homo sapiens |
| Official full symbol | myelin protein zero like 1 |
| Gene type | protein-coding |
| Also known as | MPZL1b, PZR, PZR1b, PZRa, PZRb |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MPZL1-201 | ENST00000359523.7 | 4978 | 269aa | Protein coding | CCDS1264 | O95297-1 | NM_003953.6 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| MPZL1-203 | ENST00000392121.7 | 3421 | 119aa | Protein coding | CCDS53425 | O95297-5 | - | TSL:2, GENCODE basic, |
| MPZL1-209 | ENST00000474859.5 | 630 | 209aa | Protein coding | CCDS44273 | O95297-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| MPZL1-202 | ENST00000367853.3 | 881 | 183aa | Protein coding | - | Q9UEL6 | - | CDS 5' incomplete, TSL:1, |
| MPZL1-205 | ENST00000448405.5 | 1596 | 95aa | Nonsense mediated decay | - | F8WFC4 | - | TSL:1, |
| MPZL1-204 | ENST00000403379.3 | 961 | No protein | Processed transcript | - | - | - | TSL:5, |
| MPZL1-211 | ENST00000498279.1 | 534 | No protein | Processed transcript | - | - | - | TSL:4, |
| MPZL1-210 | ENST00000487858.1 | 420 | No protein | Processed transcript | - | - | - | TSL:4, |
| MPZL1-208 | ENST00000474729.5 | 1335 | No protein | Retained intron | - | - | - | TSL:1, |
| MPZL1-207 | ENST00000465787.5 | 1305 | No protein | Retained intron | - | - | - | TSL:1, |
| MPZL1-206 | ENST00000464954.1 | 607 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MPZL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MPZL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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