CRISPR-U™ Gene Knockout Cell Line
Strategy
SELENBP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SELENBP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SELENBP1 |
| Gene id | 8991 |
| Organism | Homo sapiens |
| Official full symbol | selenium binding protein 1 |
| Gene type | protein-coding |
| Also known as | EHMTO, HEL-S-134P, LPSB, MTO, SBP56, SP56, hSBP |
| Summary | This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SELENBP1-204 | ENST00000426705.6 | 1928 | 514aa | Protein coding | CCDS60266 | Q13228-4 | - | TSL:2, GENCODE basic, |
| SELENBP1-201 | ENST00000368868.10 | 1722 | 472aa | Protein coding | CCDS995 | Q13228-1 | NM_003944.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SELENBP1-208 | ENST00000447402.7 | 1530 | 410aa | Protein coding | CCDS58027 | Q13228-3 | - | TSL:2, GENCODE basic, |
| SELENBP1-211 | ENST00000458566.5 | 929 | 295aa | Protein coding | - | A6PVX1 | - | CDS 3' incomplete, TSL:5, |
| SELENBP1-203 | ENST00000424475.5 | 916 | 305aa | Protein coding | - | H0Y532 | - | CDS 5' and 3' incomplete, TSL:5, |
| SELENBP1-205 | ENST00000427867.2 | 377 | 80aa | Protein coding | - | C9JVL0 | - | CDS 3' incomplete, TSL:5, |
| SELENBP1-202 | ENST00000423070.5 | 1887 | 165aa | Nonsense mediated decay | - | F8WCR4 | - | TSL:5, |
| SELENBP1-207 | ENST00000443708.5 | 1724 | 123aa | Nonsense mediated decay | - | F2Z2W8 | - | TSL:5, |
| SELENBP1-206 | ENST00000427977.6 | 1611 | 123aa | Nonsense mediated decay | - | F2Z2W8 | - | TSL:5, |
| SELENBP1-209 | ENST00000455397.5 | 1521 | 123aa | Nonsense mediated decay | - | F2Z2W8 | - | TSL:5, |
| SELENBP1-210 | ENST00000455839.5 | 788 | 47aa | Nonsense mediated decay | - | F8WBA9 | - | TSL:5, |
| SELENBP1-215 | ENST00000473693.5 | 722 | No protein | Processed transcript | - | - | - | TSL:5, |
| SELENBP1-219 | ENST00000498494.1 | 652 | No protein | Processed transcript | - | - | - | TSL:3, |
| SELENBP1-216 | ENST00000474352.5 | 2840 | No protein | Retained intron | - | - | - | TSL:2, |
| SELENBP1-214 | ENST00000470345.5 | 2128 | No protein | Retained intron | - | - | - | TSL:2, |
| SELENBP1-218 | ENST00000493560.5 | 1963 | No protein | Retained intron | - | - | - | TSL:5, |
| SELENBP1-212 | ENST00000463664.5 | 1922 | No protein | Retained intron | - | - | - | TSL:5, |
| SELENBP1-217 | ENST00000492643.5 | 933 | No protein | Retained intron | - | - | - | TSL:3, |
| SELENBP1-213 | ENST00000465273.1 | 342 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SELENBP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SELENBP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$49
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$599
Single gRNA lentivirus
Work flow
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