CRISPR-U™ Gene Knockout Cell Line Strategy

CCND3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CCND3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCND3
Gene id 896
Organism Homo sapiens
Official full symbol cyclin D3
Gene type protein-coding
Summary The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCND3-215 ENST00000511642.5 2394 211aa Protein coding CCDS47426 P30281-2 - TSL:2, GENCODE basic,
CCND3-201 ENST00000372987.8 2233 242aa Protein coding CCDS75452 Q5T8J1 - TSL:2, GENCODE basic,
CCND3-202 ENST00000372988.8 2133 211aa Protein coding CCDS47426 P30281-2 - TSL:1, GENCODE basic,
CCND3-223 ENST00000616010.4 2047 96aa Protein coding CCDS47427 P30281-4 - TSL:5, GENCODE basic,
CCND3-203 ENST00000372991.9 2026 292aa Protein coding CCDS4863 P30281-1 NM_001760.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CCND3-205 ENST00000415497.6 1843 96aa Protein coding CCDS47427 P30281-4 - TSL:2, GENCODE basic,
CCND3-204 ENST00000414200.6 1724 220aa Protein coding CCDS47425 P30281-3 - TSL:2, GENCODE basic,
CCND3-213 ENST00000510503.5 1257 195aa Protein coding - D6RI00 - TSL:3, GENCODE basic,
CCND3-218 ENST00000512426.5 876 175aa Protein coding - H0Y8M4 - CDS 5' incomplete, TSL:2,
CCND3-211 ENST00000508143.5 574 10aa Protein coding - A0A1D5RMS0 - CDS 3' incomplete, TSL:4,
CCND3-206 ENST00000502771.1 544 43aa Protein coding - D6RDL3 - CDS 3' incomplete, TSL:4,
CCND3-207 ENST00000505064.1 540 118aa Protein coding - D6RIX2 - CDS 3' incomplete, TSL:2,
CCND3-221 ENST00000514588.1 490 46aa Protein coding - D6RDF8 - CDS 3' incomplete, TSL:4,
CCND3-216 ENST00000511686.5 576 No protein Processed transcript - - - TSL:3,
CCND3-219 ENST00000513956.5 562 No protein Processed transcript - - - TSL:2,
CCND3-222 ENST00000612455.1 536 No protein Processed transcript - - - TSL:NA,
CCND3-220 ENST00000514382.5 476 No protein Processed transcript - - - TSL:4,
CCND3-217 ENST00000512381.1 432 No protein Processed transcript - - - TSL:3,
CCND3-210 ENST00000506555.5 308 No protein Processed transcript - - - TSL:3,
CCND3-208 ENST00000505672.5 932 No protein Retained intron - - - TSL:2,
CCND3-212 ENST00000510058.1 926 No protein Retained intron - - - TSL:2,
CCND3-209 ENST00000505884.5 592 No protein Retained intron - - - TSL:4,
CCND3-214 ENST00000511161.5 579 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCND3
This KO Strategy loading
Red Cotton™ Notes Gene CCND3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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