CRISPR-U™ Gene Knockout Cell Line
Strategy
CCND3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCND3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCND3 |
| Gene id | 896 |
| Organism | Homo sapiens |
| Official full symbol | cyclin D3 |
| Gene type | protein-coding |
| Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCND3-215 | ENST00000511642.5 | 2394 | 211aa | Protein coding | CCDS47426 | P30281-2 | - | TSL:2, GENCODE basic, |
| CCND3-201 | ENST00000372987.8 | 2233 | 242aa | Protein coding | CCDS75452 | Q5T8J1 | - | TSL:2, GENCODE basic, |
| CCND3-202 | ENST00000372988.8 | 2133 | 211aa | Protein coding | CCDS47426 | P30281-2 | - | TSL:1, GENCODE basic, |
| CCND3-223 | ENST00000616010.4 | 2047 | 96aa | Protein coding | CCDS47427 | P30281-4 | - | TSL:5, GENCODE basic, |
| CCND3-203 | ENST00000372991.9 | 2026 | 292aa | Protein coding | CCDS4863 | P30281-1 | NM_001760.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CCND3-205 | ENST00000415497.6 | 1843 | 96aa | Protein coding | CCDS47427 | P30281-4 | - | TSL:2, GENCODE basic, |
| CCND3-204 | ENST00000414200.6 | 1724 | 220aa | Protein coding | CCDS47425 | P30281-3 | - | TSL:2, GENCODE basic, |
| CCND3-213 | ENST00000510503.5 | 1257 | 195aa | Protein coding | - | D6RI00 | - | TSL:3, GENCODE basic, |
| CCND3-218 | ENST00000512426.5 | 876 | 175aa | Protein coding | - | H0Y8M4 | - | CDS 5' incomplete, TSL:2, |
| CCND3-211 | ENST00000508143.5 | 574 | 10aa | Protein coding | - | A0A1D5RMS0 | - | CDS 3' incomplete, TSL:4, |
| CCND3-206 | ENST00000502771.1 | 544 | 43aa | Protein coding | - | D6RDL3 | - | CDS 3' incomplete, TSL:4, |
| CCND3-207 | ENST00000505064.1 | 540 | 118aa | Protein coding | - | D6RIX2 | - | CDS 3' incomplete, TSL:2, |
| CCND3-221 | ENST00000514588.1 | 490 | 46aa | Protein coding | - | D6RDF8 | - | CDS 3' incomplete, TSL:4, |
| CCND3-216 | ENST00000511686.5 | 576 | No protein | Processed transcript | - | - | - | TSL:3, |
| CCND3-219 | ENST00000513956.5 | 562 | No protein | Processed transcript | - | - | - | TSL:2, |
| CCND3-222 | ENST00000612455.1 | 536 | No protein | Processed transcript | - | - | - | TSL:NA, |
| CCND3-220 | ENST00000514382.5 | 476 | No protein | Processed transcript | - | - | - | TSL:4, |
| CCND3-217 | ENST00000512381.1 | 432 | No protein | Processed transcript | - | - | - | TSL:3, |
| CCND3-210 | ENST00000506555.5 | 308 | No protein | Processed transcript | - | - | - | TSL:3, |
| CCND3-208 | ENST00000505672.5 | 932 | No protein | Retained intron | - | - | - | TSL:2, |
| CCND3-212 | ENST00000510058.1 | 926 | No protein | Retained intron | - | - | - | TSL:2, |
| CCND3-209 | ENST00000505884.5 | 592 | No protein | Retained intron | - | - | - | TSL:4, |
| CCND3-214 | ENST00000511161.5 | 579 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCND3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCND3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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