CRISPR-U™ Gene Knockout Cell Line Strategy

BTRC Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BTRC Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BTRC
Gene id 8945
Organism Homo sapiens
Official full symbol beta-transducin repeat containing E3 ubiquitin protein ligase
Gene type protein-coding
Also known as BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BTRC-203 ENST00000393441.8 6084 579aa Protein coding CCDS73183 B7Z3H4 - TSL:1, GENCODE basic,
BTRC-202 ENST00000370187.8 6024 605aa Protein coding CCDS7512 Q9Y297-1 NM_033637.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
BTRC-204 ENST00000408038.6 5982 569aa Protein coding CCDS7511 Q9Y297-2 - TSL:1, GENCODE basic, APPRIS ALT1,
BTRC-201 ENST00000370183.2 655 191aa Protein coding - Q5T1W7 - CDS 3' incomplete, TSL:3,
BTRC-207 ENST00000493877.1 772 No protein Processed transcript - - - TSL:5,
BTRC-205 ENST00000465182.1 685 No protein Processed transcript - - - TSL:3,
BTRC-206 ENST00000475200.5 527 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BTRC
This KO Strategy loading
Red Cotton™ Notes Gene BTRC had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Single gRNA lentivirus

Work flow
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