CRISPR-U™ Gene Knockout Cell Line Strategy

CCND2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CCND2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCND2
Gene id 894
Organism Homo sapiens
Official full symbol cyclin D2
Gene type protein-coding
Also known as KIAK0002, MPPH3
Summary The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).
Genomic regions Chromosome 12
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCND2-208 ENST00000676411.1 6849 289aa Protein coding CCDS8524 - - APPRIS P1,
CCND2-201 ENST00000261254.8 6493 289aa Protein coding CCDS8524 P30279-1 NM_001759.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CCND2-207 ENST00000676279.1 6352 289aa Protein coding CCDS8524 - - APPRIS P1,
CCND2-206 ENST00000675880.1 6532 303aa Protein coding - - - GENCODE basic,
CCND2-205 ENST00000675468.1 6135 No protein Processed transcript - - - -
CCND2-202 ENST00000536537.2 1062 No protein Processed transcript - - - TSL:5,
CCND2-203 ENST00000536795.1 572 No protein Retained intron - - - TSL:3,
CCND2-204 ENST00000541542.1 322 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCND2
This KO Strategy loading
Red Cotton™ Notes Gene CCND2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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