CRISPR-U™ Gene Knockout Cell Line Strategy

BCL10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCL10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCL10
Gene id 8915
Organism Homo sapiens
Official full symbol BCL10 immune signaling adaptor
Gene type protein-coding
Also known as CARMEN, CIPER, CLAP, IMD37, c-E10, mE10
Summary This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCL10-202 ENST00000648566.1 2833 233aa Protein coding CCDS704 O95999 NM_003921.5 GENCODE basic, APPRIS P1, MANE Select v0.92,
BCL10-201 ENST00000620248.2 776 222aa Protein coding - A0A087WWW9 - TSL:5, GENCODE basic,
BCL10-203 ENST00000649060.1 1561 80aa Nonsense mediated decay - A0A3B3ISX2 - -
BCL10-205 ENST00000650582.1 1131 No protein Processed transcript - - - -
BCL10-204 ENST00000649434.1 573 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCL10
This KO Strategy loading
Red Cotton™ Notes Gene BCL10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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