CRISPR-U™ Gene Knockout Cell Line
Strategy
BCL10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCL10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCL10 |
| Gene id | 8915 |
| Organism | Homo sapiens |
| Official full symbol | BCL10 immune signaling adaptor |
| Gene type | protein-coding |
| Also known as | CARMEN, CIPER, CLAP, IMD37, c-E10, mE10 |
| Summary | This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCL10-202 | ENST00000648566.1 | 2833 | 233aa | Protein coding | CCDS704 | O95999 | NM_003921.5 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BCL10-201 | ENST00000620248.2 | 776 | 222aa | Protein coding | - | A0A087WWW9 | - | TSL:5, GENCODE basic, |
| BCL10-203 | ENST00000649060.1 | 1561 | 80aa | Nonsense mediated decay | - | A0A3B3ISX2 | - | - |
| BCL10-205 | ENST00000650582.1 | 1131 | No protein | Processed transcript | - | - | - | - |
| BCL10-204 | ENST00000649434.1 | 573 | No protein | Processed transcript | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCL10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCL10 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: