CRISPR-U™ Gene Knockout Cell Line Strategy

CDC16 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDC16 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC16
Gene id 8881
Organism Homo sapiens
Official full symbol cell division cycle 16
Gene type protein-coding
Also known as ANAPC6, APC6, CDC16Hs, CUT9
Summary The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified.
Genomic regions Chromosome 13
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC16-203 ENST00000356221.8 2331 620aa Protein coding CCDS9542 Q13042-1 NM_001078645.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CDC16-204 ENST00000360383.7 2211 620aa Protein coding CCDS9542 Q13042-1 - TSL:5, GENCODE basic, APPRIS P4,
CDC16-206 ENST00000375310.5 2156 526aa Protein coding CCDS81787 Q13042-4 - TSL:1, GENCODE basic,
CDC16-201 ENST00000252457.9 2034 619aa Protein coding CCDS81786 Q13042-2 - TSL:1, GENCODE basic, APPRIS ALT1,
CDC16-205 ENST00000375308.5 2034 526aa Protein coding CCDS81787 Q13042-4 - TSL:1, GENCODE basic,
CDC16-211 ENST00000628084.2 2058 475aa Protein coding - Q5T8C6 - TSL:5, GENCODE basic,
CDC16-202 ENST00000252458.6 1991 475aa Protein coding - Q5T8C6 - TSL:1, GENCODE basic,
CDC16-212 ENST00000650489.1 2204 539aa Nonsense mediated decay - A0A3B3ISD2 - -
CDC16-207 ENST00000461716.1 700 No protein Processed transcript - - - TSL:5,
CDC16-209 ENST00000494581.1 614 No protein Processed transcript - - - TSL:3,
CDC16-208 ENST00000484907.1 1038 No protein Retained intron - - - TSL:3,
CDC16-210 ENST00000494766.5 1003 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC16
This KO Strategy loading
Red Cotton™ Notes Gene CDC16 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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