CRISPR-U™ Gene Knockout Cell Line Strategy

DPM2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DPM2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DPM2
Gene id 8818
Organism Homo sapiens
Official full symbol dolichyl-phosphate mannosyltransferase subunit 2, regulatory
Gene type protein-coding
Also known as CDG1U
Summary Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1.
Genomic regions Chromosome 9
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DPM2-201 ENST00000314392.13 912 84aa Protein coding CCDS6886 O94777 NM_003863.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DPM2-202 ENST00000373110.4 738 130aa Protein coding - Q5T9C7 - TSL:2, GENCODE basic,
DPM2-205 ENST00000495270.1 1662 No protein Retained intron - - - TSL:1,
DPM2-203 ENST00000470181.1 928 No protein Retained intron - - - TSL:1,
DPM2-204 ENST00000473360.1 744 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DPM2
This KO Strategy loading
Red Cotton™ Notes Gene DPM2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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