CRISPR-U™ Gene Knockout Cell Line
Strategy
DPM2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DPM2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DPM2 |
| Gene id | 8818 |
| Organism | Homo sapiens |
| Official full symbol | dolichyl-phosphate mannosyltransferase subunit 2, regulatory |
| Gene type | protein-coding |
| Also known as | CDG1U |
| Summary | Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DPM2-201 | ENST00000314392.13 | 912 | 84aa | Protein coding | CCDS6886 | O94777 | NM_003863.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DPM2-202 | ENST00000373110.4 | 738 | 130aa | Protein coding | - | Q5T9C7 | - | TSL:2, GENCODE basic, |
| DPM2-205 | ENST00000495270.1 | 1662 | No protein | Retained intron | - | - | - | TSL:1, |
| DPM2-203 | ENST00000470181.1 | 928 | No protein | Retained intron | - | - | - | TSL:1, |
| DPM2-204 | ENST00000473360.1 | 744 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DPM2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DPM2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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