CRISPR-U™ Gene Knockout Cell Line Strategy

CDKL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDKL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDKL1
Gene id 8814
Organism Homo sapiens
Official full symbol cyclin dependent kinase like 1
Gene type protein-coding
Also known as KKIALRE, P42
Summary This gene product is a member of a large family of CDC2-related serine/threonine protein kinases that accumulates primarily in the nucleus.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDKL1-201 ENST00000216378.2 2209 276aa Protein coding CCDS73637 A0A5H1ZRP5 - TSL:1, GENCODE basic,
CDKL1-203 ENST00000395834.6 5402 357aa Protein coding - Q00532 NM_004196.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDKL1-209 ENST00000534267.5 745 140aa Protein coding - H0YE65 - CDS 5' incomplete, TSL:2,
CDKL1-204 ENST00000525911.5 210 70aa Protein coding - H0YCE5 - CDS 5' and 3' incomplete, TSL:3,
CDKL1-202 ENST00000356146.5 2982 No protein Processed transcript - - - TSL:1,
CDKL1-208 ENST00000531052.1 704 No protein Processed transcript - - - TSL:3,
CDKL1-205 ENST00000528197.5 573 No protein Processed transcript - - - TSL:4,
CDKL1-211 ENST00000542671.5 487 No protein Processed transcript - - - TSL:5,
CDKL1-207 ENST00000530553.1 311 No protein Processed transcript - - - TSL:3,
CDKL1-210 ENST00000534566.1 192 No protein Processed transcript - - - TSL:2,
CDKL1-206 ENST00000529347.1 673 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDKL1
This KO Strategy loading
Red Cotton™ Notes Gene CDKL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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