CRISPR-U™ Gene Knockout Cell Line
Strategy
CDKL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDKL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDKL1 |
| Gene id | 8814 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase like 1 |
| Gene type | protein-coding |
| Also known as | KKIALRE, P42 |
| Summary | This gene product is a member of a large family of CDC2-related serine/threonine protein kinases that accumulates primarily in the nucleus. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDKL1-201 | ENST00000216378.2 | 2209 | 276aa | Protein coding | CCDS73637 | A0A5H1ZRP5 | - | TSL:1, GENCODE basic, |
| CDKL1-203 | ENST00000395834.6 | 5402 | 357aa | Protein coding | - | Q00532 | NM_004196.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDKL1-209 | ENST00000534267.5 | 745 | 140aa | Protein coding | - | H0YE65 | - | CDS 5' incomplete, TSL:2, |
| CDKL1-204 | ENST00000525911.5 | 210 | 70aa | Protein coding | - | H0YCE5 | - | CDS 5' and 3' incomplete, TSL:3, |
| CDKL1-202 | ENST00000356146.5 | 2982 | No protein | Processed transcript | - | - | - | TSL:1, |
| CDKL1-208 | ENST00000531052.1 | 704 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDKL1-205 | ENST00000528197.5 | 573 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDKL1-211 | ENST00000542671.5 | 487 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDKL1-207 | ENST00000530553.1 | 311 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDKL1-210 | ENST00000534566.1 | 192 | No protein | Processed transcript | - | - | - | TSL:2, |
| CDKL1-206 | ENST00000529347.1 | 673 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDKL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDKL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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