CRISPR-U™ Gene Knockout Cell Line
Strategy
TRIM24 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRIM24 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TRIM24 |
| Gene id | 8805 |
| Organism | Homo sapiens |
| Official full symbol | tripartite motif containing 24 |
| Gene type | protein-coding |
| Also known as | PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 |
| Summary | The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TRIM24-201 | ENST00000343526.9 | 8488 | 1050aa | Protein coding | CCDS5847 | O15164-1 | NM_015905.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| TRIM24-202 | ENST00000415680.6 | 3799 | 1016aa | Protein coding | CCDS47720 | O15164-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| TRIM24-206 | ENST00000497516.5 | 1515 | No protein | Processed transcript | - | - | - | TSL:5, |
| TRIM24-205 | ENST00000493595.1 | 811 | No protein | Processed transcript | - | - | - | TSL:3, |
| TRIM24-203 | ENST00000464210.1 | 545 | No protein | Processed transcript | - | - | - | TSL:4, |
| TRIM24-204 | ENST00000485318.1 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TRIM24 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TRIM24 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: