CRISPR-U™ Gene Knockout Cell Line
Strategy
DYRK4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DYRK4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DYRK4 |
| Gene id | 8798 |
| Organism | Homo sapiens |
| Official full symbol | dual specificity tyrosine phosphorylation regulated kinase 4 |
| Gene type | protein-coding |
| Summary | This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DYRK4-211 | ENST00000540757.6 | 3272 | 520aa | Protein coding | CCDS8530 | Q9NR20-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| DYRK4-201 | ENST00000010132.6 | 1840 | 520aa | Protein coding | CCDS8530 | Q9NR20-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| DYRK4-214 | ENST00000543431.5 | 2068 | 634aa | Protein coding | - | A0A0A0MTH5 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| DYRK4-216 | ENST00000544671.1 | 470 | 120aa | Protein coding | - | H0YFI4 | - | CDS 5' incomplete, TSL:2, |
| DYRK4-207 | ENST00000539309.5 | 989 | 43aa | Nonsense mediated decay | - | F5H3D3 | - | TSL:5, |
| DYRK4-209 | ENST00000539701.5 | 945 | 68aa | Nonsense mediated decay | - | F5GWS4 | - | TSL:5, |
| DYRK4-205 | ENST00000537719.5 | 454 | 59aa | Nonsense mediated decay | - | F5H4X6 | - | TSL:3, |
| DYRK4-208 | ENST00000539490.1 | 447 | No protein | Processed transcript | - | - | - | TSL:2, |
| DYRK4-204 | ENST00000536645.1 | 415 | No protein | Processed transcript | - | - | - | TSL:3, |
| DYRK4-203 | ENST00000536157.5 | 1519 | No protein | Retained intron | - | - | - | TSL:1, |
| DYRK4-213 | ENST00000542905.1 | 985 | No protein | Retained intron | - | - | - | TSL:1, |
| DYRK4-217 | ENST00000545571.5 | 855 | No protein | Retained intron | - | - | - | TSL:1, |
| DYRK4-212 | ENST00000541024.1 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
| DYRK4-202 | ENST00000536137.1 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| DYRK4-215 | ENST00000544050.1 | 514 | No protein | Retained intron | - | - | - | TSL:2, |
| DYRK4-206 | ENST00000538520.5 | 440 | No protein | Retained intron | - | - | - | TSL:3, |
| DYRK4-210 | ENST00000540644.1 | 400 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DYRK4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DYRK4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$599
Single gRNA lentivirus
Work flow
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