CRISPR-U™ Gene Knockout Cell Line Strategy

DYRK4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DYRK4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DYRK4
Gene id 8798
Organism Homo sapiens
Official full symbol dual specificity tyrosine phosphorylation regulated kinase 4
Gene type protein-coding
Summary This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Genomic regions Chromosome 12
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DYRK4-211 ENST00000540757.6 3272 520aa Protein coding CCDS8530 Q9NR20-1 - TSL:1, GENCODE basic, APPRIS P4,
DYRK4-201 ENST00000010132.6 1840 520aa Protein coding CCDS8530 Q9NR20-1 - TSL:5, GENCODE basic, APPRIS P4,
DYRK4-214 ENST00000543431.5 2068 634aa Protein coding - A0A0A0MTH5 - TSL:5, GENCODE basic, APPRIS ALT2,
DYRK4-216 ENST00000544671.1 470 120aa Protein coding - H0YFI4 - CDS 5' incomplete, TSL:2,
DYRK4-207 ENST00000539309.5 989 43aa Nonsense mediated decay - F5H3D3 - TSL:5,
DYRK4-209 ENST00000539701.5 945 68aa Nonsense mediated decay - F5GWS4 - TSL:5,
DYRK4-205 ENST00000537719.5 454 59aa Nonsense mediated decay - F5H4X6 - TSL:3,
DYRK4-208 ENST00000539490.1 447 No protein Processed transcript - - - TSL:2,
DYRK4-204 ENST00000536645.1 415 No protein Processed transcript - - - TSL:3,
DYRK4-203 ENST00000536157.5 1519 No protein Retained intron - - - TSL:1,
DYRK4-213 ENST00000542905.1 985 No protein Retained intron - - - TSL:1,
DYRK4-217 ENST00000545571.5 855 No protein Retained intron - - - TSL:1,
DYRK4-212 ENST00000541024.1 587 No protein Retained intron - - - TSL:2,
DYRK4-202 ENST00000536137.1 560 No protein Retained intron - - - TSL:2,
DYRK4-215 ENST00000544050.1 514 No protein Retained intron - - - TSL:2,
DYRK4-206 ENST00000538520.5 440 No protein Retained intron - - - TSL:3,
DYRK4-210 ENST00000540644.1 400 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DYRK4
This KO Strategy loading
Red Cotton™ Notes Gene DYRK4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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