CRISPR-U™ Gene Knockout Cell Line Strategy

TNFRSF10B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TNFRSF10B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TNFRSF10B
Gene id 8795
Organism Homo sapiens
Official full symbol TNF receptor superfamily member 10b
Gene type protein-coding
Also known as CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9
Summary The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.
Genomic regions Chromosome 8
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TNFRSF10B-201 ENST00000276431.9 3998 440aa Protein coding CCDS6035 O14763-1 NM_003842.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
TNFRSF10B-202 ENST00000347739.3 1723 411aa Protein coding CCDS6036 O14763-2 - TSL:1, GENCODE basic, APPRIS ALT2,
TNFRSF10B-207 ENST00000523504.5 1464 90aa Nonsense mediated decay - E9PBT3 - TSL:2,
TNFRSF10B-205 ENST00000519910.1 546 No protein Processed transcript - - - TSL:4,
TNFRSF10B-208 ENST00000523752.5 3328 No protein Retained intron - - - TSL:1,
TNFRSF10B-206 ENST00000520109.1 674 No protein Retained intron - - - TSL:2,
TNFRSF10B-204 ENST00000519028.1 612 No protein Retained intron - - - TSL:2,
TNFRSF10B-203 ENST00000518531.5 564 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TNFRSF10B
This KO Strategy loading
Red Cotton™ Notes Gene TNFRSF10B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Single gRNA plasmid off-shelf

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Single gRNA lentivirus

Work flow
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