CRISPR-U™ Gene Knockout Cell Line
Strategy
FADD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FADD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FADD |
| Gene id | 8772 |
| Organism | Homo sapiens |
| Official full symbol | Fas associated via death domain |
| Gene type | protein-coding |
| Also known as | GIG3, MORT1 |
| Summary | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FADD-201 | ENST00000301838.5 | 1708 | 208aa | Protein coding | CCDS8196 | Q13158 | NM_003824.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FADD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FADD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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