CRISPR-U™ Gene Knockout Cell Line
Strategy
RIPK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RIPK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RIPK2 |
| Gene id | 8767 |
| Organism | Homo sapiens |
| Official full symbol | receptor interacting serine/threonine kinase 2 |
| Gene type | protein-coding |
| Also known as | CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 |
| Summary | This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RIPK2-201 | ENST00000220751.5 | 2516 | 540aa | Protein coding | CCDS6247 | O43353-1 | NM_003821.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RIPK2-204 | ENST00000522965.1 | 1916 | 68aa | Nonsense mediated decay | - | E7ERW9 | - | TSL:1, |
| RIPK2-202 | ENST00000517696.1 | 572 | 70aa | Nonsense mediated decay | - | E5RGK6 | - | TSL:4, |
| RIPK2-203 | ENST00000518673.1 | 481 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RIPK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RIPK2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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