CRISPR-U™ Gene Knockout Cell Line Strategy

RIPK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RIPK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RIPK2
Gene id 8767
Organism Homo sapiens
Official full symbol receptor interacting serine/threonine kinase 2
Gene type protein-coding
Also known as CARD3, CARDIAK, CCK, GIG30, RICK, RIP2
Summary This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.
Genomic regions Chromosome 8
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RIPK2-201 ENST00000220751.5 2516 540aa Protein coding CCDS6247 O43353-1 NM_003821.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RIPK2-204 ENST00000522965.1 1916 68aa Nonsense mediated decay - E7ERW9 - TSL:1,
RIPK2-202 ENST00000517696.1 572 70aa Nonsense mediated decay - E5RGK6 - TSL:4,
RIPK2-203 ENST00000518673.1 481 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RIPK2
This KO Strategy loading
Red Cotton™ Notes Gene RIPK2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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