CRISPR-U™ Gene Knockout Cell Line
Strategy
B4GALT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human B4GALT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | B4GALT2 |
| Gene id | 8704 |
| Organism | Homo sapiens |
| Official full symbol | beta-1,4-galactosyltransferase 2 |
| Gene type | protein-coding |
| Also known as | B4Gal-T2, B4Gal-T3, beta4Gal-T2 |
| Summary | This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| B4GALT2-202 | ENST00000356836.10 | 2629 | 372aa | Protein coding | CCDS506 | O60909-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| B4GALT2-204 | ENST00000434555.7 | 2271 | 372aa | Protein coding | CCDS506 | O60909-2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| B4GALT2-203 | ENST00000372324.6 | 2193 | 372aa | Protein coding | CCDS506 | O60909-1 | NM_003780.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| B4GALT2-201 | ENST00000309519.8 | 2004 | 401aa | Protein coding | CCDS55596 | O60909-3 | - | TSL:2, GENCODE basic, |
| B4GALT2-205 | ENST00000481924.2 | 3443 | 323aa | Protein coding | - | - | - | TSL:2, GENCODE basic, |
| B4GALT2-208 | ENST00000498543.1 | 360 | 55aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
| B4GALT2-206 | ENST00000485678.6 | 1932 | 216aa | Nonsense mediated decay | - | - | - | TSL:3, |
| B4GALT2-207 | ENST00000497866.6 | 1923 | 136aa | Nonsense mediated decay | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | B4GALT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene B4GALT2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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