CRISPR-U™ Gene Knockout Cell Line Strategy

B4GALT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human B4GALT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol B4GALT2
Gene id 8704
Organism Homo sapiens
Official full symbol beta-1,4-galactosyltransferase 2
Gene type protein-coding
Also known as B4Gal-T2, B4Gal-T3, beta4Gal-T2
Summary This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
B4GALT2-202 ENST00000356836.10 2629 372aa Protein coding CCDS506 O60909-1 - TSL:2, GENCODE basic, APPRIS P1,
B4GALT2-204 ENST00000434555.7 2271 372aa Protein coding CCDS506 O60909-2 - TSL:1, GENCODE basic, APPRIS P1,
B4GALT2-203 ENST00000372324.6 2193 372aa Protein coding CCDS506 O60909-1 NM_003780.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
B4GALT2-201 ENST00000309519.8 2004 401aa Protein coding CCDS55596 O60909-3 - TSL:2, GENCODE basic,
B4GALT2-205 ENST00000481924.2 3443 323aa Protein coding - - - TSL:2, GENCODE basic,
B4GALT2-208 ENST00000498543.1 360 55aa Protein coding - - - CDS 3' incomplete, TSL:3,
B4GALT2-206 ENST00000485678.6 1932 216aa Nonsense mediated decay - - - TSL:3,
B4GALT2-207 ENST00000497866.6 1923 136aa Nonsense mediated decay - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene B4GALT2
This KO Strategy loading
Red Cotton™ Notes Gene B4GALT2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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